Toll样受体9 (TLR9) 可识别特定的未甲基化的CpG-ODN序列（该序列只存在于微生物DNA而不存在于哺乳动物的DNA），诱导天然免疫应答和获得性免疫应答。微生物DNA的免疫刺激效应可由合成的包含这些CpG ODNs的寡核苷酸模拟。
根据结构不同和对人外周血单个核细胞（PBMC）、特定B细胞和浆样树突状细胞的活性不同，CpG ODNs可分为A、B、C三型。
● CpG-A ODNs 的特征为一个PO中心 CpG包含性回文结构和一个PS修饰的3’ poly-G, 可诱导浆样树突状细胞产生高水平的IFN-α，但对TLR9依赖性NF-κB信号和促炎因子（如IL-6）的刺激作用较弱。
● CpG-B ODNs 包含一个完整的PS骨架和一个或多个CpG二核苷酸， 可强力有效的活化B细胞和TLR9依赖性NF-κB信号，但刺激IFN-α 产生的作用较弱。
● CpG-C ODNs 既具有A型的特征也具有B型的特征，在结构上含有一个完整的PS骨架和一个CpG包含性回文结构，可有效的诱导浆样树突状细胞产生IFN-α和有效刺激B细胞的应答。
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Class
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Backbone
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B cells
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pDCs
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IFN-α
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Il-6
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A
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PS-PO
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+
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++++
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++++
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+
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B
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PS
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++++
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++++
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+
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+++
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C
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PS
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+++
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+++
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+++
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++
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选择正确的CpG ODN类型
| TLR9 激动剂 | |||
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Class A
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TLR9 Agonist - Stimulatory CpG ODN, Class A, Mouse specific
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TLR9 Agonist - Stimulatory CpG ODN, Class A, Human specific
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Strips pre-coated with ODN 2216, a TLR9 agonist
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TLR9 Agonist - Stimulatory CpG ODN, Class A, Human specific
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Class B
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TLR9 Agonist - Stimulatory CpG ODN, Class B, Human & mouse
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TLR9 Agonist - Stimulatory CpG ODN, Class B, Multispecies
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TLR9 Agonist - Stimulatory CpG ODN, Class B, Mouse specific
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TLR9 Agonist - Stimulatory CpG ODN, Class B, Mouse specific
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TLR9 Agonist - Stimulatory CpG ODN, Class B, Human specific
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Strips pre-coated with ODN 2006, a TLR9 agonist
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TLR9 Agonist - Stimulatory CpG ODN, Class B, Bovine / porcine
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Set of known multispecies TLR9 agonists (B-Class CpG ODNs)
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Class C
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TLR9 Agonist - Stimulatory CpG ODN, Class C, Multispecies
TLR9 ligand |
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TLR9 Agonist - Stimulatory CpG ODN, Class C, Human / mouse
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TLR9 Agonist - Stimulatory CpG ODN, Class C, Human / mouse
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Bacterial DNA
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TLR9 Agonist - DNA from E. coli
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TLR9 Ligand Kits
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Set of known agonists for human TLR9
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Set of known agonists for mouse TLR9
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Set of known multispecies TLR9 agonists (B-Class CpG ODNs)
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Set of known multispecies TLR9 agonists (A & C-Classes CpG
ODNs) |
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Set of known antagonists for human TLR9
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| TLR9 拮抗剂 | |
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TLR9 Antagonist - Inhibitory ODN, preference towards mouse
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TLR9 Antagonist - Negative control for ODN 2088
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TLR9 Antagonist - Class B inhibitory ODN
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TLR9 Antagonist - Class R inhibitory ODN
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TLR9 antagonist - Class B inhibitory ODN oligonucleotide
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TLR9 Antagonist - Negative control for ODN INH-1
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TLR9 Antagonist - Inhibitory ODN, preference towards human
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TLR9 Antagonist - Negative control for ODN TTAGGG
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TLR9 Antagonist - Inhibitory guanosine-rich ODN
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Negative control for guanosine-rich inhibitory oligonucleotide (G-
ODN) |
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