Cayman检测试剂盒(Assay kits)---线粒体研究/转录因子分析篇

线粒体研究(Mitochondria Research)

线粒体位于细胞核外, 既能产生能量又携带遗传信息,其结构的特殊性决定其损伤及修复的方式与核DNA有所不同。检测线粒体呼吸链功能对于诊断线粒体疾病是必要的。Cayman公司开发了线粒体复合体活性检测试剂盒以及氧消耗率检测试剂盒。

产品名称	编号	规格
MitoCheck Complex I Activity Assay Kit	700930	96 wells
MitoCheck Complex II Activity Assay Kit	700940	96 wells
MitoCheck Complex II/III Activity Assay Kit	700950	96 wells
Oxygen Consumption Rate Assay Kit(MitoXpress-Xtra HS Method)	600800	96 wells
Oxygen Consumption/MitoMembrane Potential Dual Assay Kit	600880	96 wells

转录因子分析 (Transcription Factor Assays)

Cayman研发的转录因子分析技术，采用非放射性、高灵敏度的方法，检测细胞核提取物中转录因子与DNA的特异性结合活性。该技术取代传统繁杂的EMSA方法，用96孔板形式来分析转录因子与DNA的结合。主要技术原理为，首先将特异性dsDNA序列(含应答元件)包被固定于96孔板中，再将含转录因子的核提取物加入板孔中孵育，转录因子与包被的DNA序列特异性结合，然后用转录因子特异性的一抗孵育，最后加入相应的二抗-HRP结合物，显色并于450nm读数。

产品名称	编号	规格
ATF2(Phospho-Thr69,71) Transcription Factor Assay Kit	600130	96 wells
ChREBP Cell-Based Translocation Assay Kit	10010060	96 wells
ChREBP Transcription Factor Assay Kit	10006909	96 wells
CREB(Phospho-Ser133)Transcription Factor Assay Kit	10009846	96 wells
HIF-1alpha Transcription Factor Assay Kit	10006910	96 wells
NF-kB(human p50) Transcription Factor Assay Kit	10006912	96 wells
NF-kB(p65) Transcription Factor Assay Kit	10007889	96 wells
NF-kB(p50/p65) Combo Transcription Factor Assay Kit	10011223	96 wells
Nrf2 Transcription Factor Assay Kit	600590	96 wells
p53 Cell-Based Activation/Translocation Assay Kit	600008	96 wells
p53 Designer Transcription Factor Assay Kit	600030	96 wells
p53 total and p53(Phospho-Ser392) Dual Staining Assay Kit	600060	96 wells
p53 Transcription Factor Assay Kit	600020	96 wells
PPARα Transcription Factor Assay Kit	10006915	96 wells
PPARδ Transcription Factor Assay Kit	10006914	96 wells
PPARγ Transcription Factor Assay Kit	10006855	96 wells
PPARα,δ,γ Complete Transcription Factor Assay Kit	10008878	96 wells
PPARγ Ligand Screening Assay Kit	10007685	96 wells
SREBP-1 Transcription Factor Assay Kit	10010854	96 wells
SREBP-2 Cell-Based Translocation Assay Kit	10009239	96 wells
SREBP-2 Transcription Factor Assay Kit	10007819	96 wells
Nuclear Extraction Kit	10009277	1 ea