天然免疫学热点领域科研抗体(AbD Serotec®)---巨噬细胞标志物抗体

AbD Serotec公司是全球领先的巨噬细胞标志物抗体供应商，其生产的MCA497, Rat Anti Mouse F4/80 Clone CI:A3-1是著名的成熟小鼠巨噬细胞表面特异性标志物抗体，有将近400篇的文献引用，提供多达30种的包装形式，可用于FC、WB，IF、IHC等7种应用。AbD Serotec享有该克隆的专利，是该抗体唯一的商业化生产商。
MCA1957识别小鼠细胞表面macrosialin，为人CD68的同源物，是专门表达于组织巨噬细胞的一种重度糖基化的跨膜糖蛋白。

小鼠淋巴结的免疫荧光染色；巨噬细胞用anti 小鼠脾脏冰冻切片的免疫组化染色；红髓中的
F4/80(MCA497PE)染成红色，B细胞用anti C- 巨噬细胞用anti CD68(MCA1957)染色
D79b(MCA1821A488)染成绿色，细胞核用D-
API复染，呈蓝色

AbD Serotec公司提供的最受欢迎的巨噬细胞标志物抗体

人巨噬细胞标志物抗体
产品名称	克隆号		产品编号	形式
CD11b	ICRF44		MCA551	Pur., A647, A700, Az. Fr., Biot., FITC, RPE, RPE-A647
CD14	UCHM1		MCA596	Pur., A647, APC, Az. Fr., Biot., FITC, RPE
CD14	TUK4		MCA1568	Pur., A647, A700, Biot., FITC, LE, PB, RPE, RPE-A647, RPE-A750
CD14	MEM-18		MCA2185	Pur., FITC, RPE
CD14	61D3		MCA2804	RPE-Cy5, RPE-Cy5.5
CD163	EDHu-1		MCA1853	Pur., FITC
CD169	7-239		MCA2517	Pur., A488, A647, FITC, LE, RPE
CD200R	OX108		MCA2282	Pur., A488, A647, Biot., FITC, RPE
CD204	Poly		AHP563	Serum
CD206	15-2		MCA2155	Pur.
CD209	MR-1		MCA2318	Pur., A488, A647, Biot., FITC, LE, RPE
CD31	WM59		MCA1738	Pur., A647, Biot., FITC, RPE
CD36	SMØ		MCA722	Pur., A647, Az. Fr., FITC
CD68	514H12		MCA1815	S/N
CD68	KiM7		MCA2375F	FITC
Dectin-1	BD6		MCA4662	Pur., A488, A647, FITC, RPE
Dectin-1	GE2		MCA4661	Pur., A488, A647, FITC, LE, RPE
Macrophage	MAC387		MCA874	Pur., A488, FITC, LE, RPE
小鼠巨噬细胞标志物抗体
CD11b		M1/70.15	MCA74	Pur., A488, A647, A700, Biot., FITC, LE, RPE, RPE- A647, RPE-A750, RPE-Cy5
CD11b		5C6	MCA711	Pur., FITC, LE, RPE
CD115		604B5	2E11	MCA1898 Pur., Az. Fr., RPE
CD169		MOMA-1	MCA947	Pur., S/N, A647, FITC
CD169		3D6.112	MCA884	Pur., FITC, LE
CD200R		OX-110	MCA2281	Pur., A488, A647, Biot., FITC, LE, RPE
CD204		2F8	MCA1322	Pur., A488, A647, Biot., FITC, LE, RPE
CD206		MR5D3	MCA2235	Pur., A488, A647, Biot., FITC, LE, RPE
CD31		ER-MP12	MCA2388	Pur., A488, A647, Biot., RPE, RPE-A647
CD36		MF3	MCA2748	Pur., A488, A647, FITC, LE, RPE
CD68		FA-11	MCA1957	Pur., A488, A647, A700, Az. Fr., Biot., FITC, RPE
Dectin-1		2A11	MCA2289	Pur., A647, Biot., FITC, LE, RPE
Dectin-2		D2.11E4	MCA2415	Pur., A488, A647, Biot., FITC, LE, RPE
F4/80		CI:A3-1	MCA497	Pur., A488, A647, A700, APC, Biot., FITC, LE, PB, RPE, RPE-A647, RPE-A750, S/N
Ly-6B.2		7/4	MCA771	Pur., A647, A700, Biot., FITC, LE, RPE
Ly-6C		ER-MP20	MCA2389	Pur., A488, A647, Biot., RPE
MARCO		ED31	MCA1849	Pur., FITC, RPE
TREM-1		L5-B8.2A12.3A12	MCA2578	Pur., A488, A647, FITC, LE, RPE
TREM-2		78.18	MCA4772	Pur., FITC, LE
大鼠巨噬细胞标志物抗体
CD11b		OX-42	MCA275	Pur., A488, A647, A700, Biot., FITC, LE, PB, RPE, RPE- A647
CD11b		ED7	MCA618R	Pur.
CD11b		ED8	MCA619	Pur., FITC, RPE
CD115		604B52E11	MCA1898	Pur., Az. Fr., RPE
CD163		ED2	MCA342	Pur., A647, Biot., FITC, LE, RPE
CD169		ED3	MCA343	Pur.
CD172a		OX41	MCA274	Pur., Az. Fr., Biot, FITC, RPE
CD172a		ED9	MCA620	Pur., FITC, RPE
CD200R1		OX-102	MCA1959	Pur., Biot., FITC, LE, RPE
CD31		TLD-3A12	MCA1334	Pur., A488, A647, Biot., FITC, LE, RPE
CD68		ED1	MCA341	Pur., A488, A647, A700, Biot., FITC, RPE

荧光多色染色试剂

产品名称	克隆号	产品编号	形式
Human CD45/CD14	F10-89-4/UCHM1	DC008	FITC/RPE
Mouse IgG2a/IgG2a	Negative Control	DC013	FITC/RPE
Mouse Ly6C/CD31	ERMP20/ERMP12	DC053	A488/RPE
Mouse Ly6C/CD31	ERMP20/ERMP12	DC054	A488/RPE-A647
Mouse CD11b/CD115	M1/70.15 / 604B5 2E11	DC055	A488/RPE
Mouse Ly6C/CD115/CD11b	ERMP20/604B52E11/M1/70.15	TC015	A488/RPE/RPE-A647
Mouse Ly6C/CD115/CD31	ERMP20/604B52E11/ERMP12	TC016	A488/RPE/RPE-A647
Mouse Ly6C/CD115/CD31	ERMP20/604B52E11/ERMP12	TC017	A488/RPE/A647
Mouse Ly6C/CD115/CD11b/CD31	ERMP20/604B52E11/M1/70.15/ERMP12	QC001	A488/RPE/RPE-A647/A647

AbD Serotec公司 F4/80 产品参考文献
1. Austyn, J.M. and Gordon, S. (1981) F4/80 , a monoclonal antibody directed specifically against the mouse macrophage.Eur. J. Immunol. 11: 805-815.
2. Hume, D.A., Perry, V.H., and Gordon, S. (1984) The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen F4/80: Macrophages Associated with Epithelia.The Anatomical Record 210: 503-512.
3. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.J. Exp. Med. 161: 475-489.
4. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
Curr. Top. Micro. Immunol. 181: 1-37.
5. Uaesoontrachoon, K. et al. (2013) Osteopontin deficiency delays inflammatory infiltration and the onset of muscle regeneration in a mouse model of muscle injury.Dis Model Mech. 6: 197-205.
6. Lin, H. H.et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.J. Exp. Med. 201: 1615–1625.
7. Chan, R.J. et al. (2005) Human somatic PTPN11 mutations induce hematopoietic cell hypersensitivity to granulocyte-macrophage colony stimulating factor.Blood. 105: 3737-3742.
8. Pizza, F.X. et al. (2005) Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice.J. Physiol. 562:899-913.
9. Tarallo, V. et al. (2011) The biflavonoid amentoflavone inhibits neovascularization preventing the activity of proangiogenic vascular endothelial growth factors.J Biol Chem. 286: 19641-51.
10. Rivollier, A. et al. (2012) Inflammation switches the differentiation program of Ly6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon.
J Exp Med. 209: 139-55.
11. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member,Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linkedmarker for subsets of mouse macrophages and dendritic cells.J Immunol. 182:1278-86.
12. Seitz, O. et al. (2010) Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study.Exp Diabetes Res. 2010: 476969.
13. Miao, E.A. et al. (2011) Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria.Nat Immunol. 11: 1136-42.
14. Wang, X. et al. (2011) Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance.Endocrinology. 152: 836-46.
15. Cunningham, O. et al. (2009) Microglia and the urokinase plasminogen activator receptor/uPA system ininnate brain inflammation.Glia. 57: 1802-14.
16. Gornicka, A. et al. (2012) Adipocyte hypertrophy is associated with lysosomal permeability both in vivo and in vitro: role in adipose tissue inflammation.Am J Physiol Endocrinol Metab. 303: E597-606.
17. Akbarshahi, H. et al. (2012) Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.PLoS One. 7: e42654.
18. Banda, N.K. et al. (1012) Role of C3a receptors, C5a receptors, and complement protein C6 deficiency in collagen antibody-induced arthritis in mice.J Immunol. 188: 1469-78.
19. Bonde, A.K. et al. (2012) Intratumoral macrophages contribute to epithelial-mesenchymal transition in solid tumors.BMC Cancer. 12: 35.
20. Choi, K.M. et al. (2010) CD206-positive M2 macrophages that express heme oxygenase-1 protect against diabetic gastroparesis in mice.Gastroenterology. 138: 2399-409,
21. Tamaki, S. et al. (2013) Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction.PLoS One. 8: e68893.
22. Kihira, Y. et al. (2014) Deletion of hypoxia-inducible factor-1a in adipocytes enhances glucagon-like Peptide-1 secretion and reduces adipose tissue inflammation.PLoS One. 9(4):e93856.