Systemic injection of TLR1/2 agonist improves adoptive
antigen-specific T cell therapy in glioma-bearing
mice
Yufei Zhang … Yiwei Chu, Clinical Immunology | 06/11/2014
... Flow cytometry analysis. GL261 tumor cells (1×10 6 ) were stained with directly conjugated antibodies: anti-TLR1, -TLR2, APC-Annexin V and PE-conjugated PI, and also with appropriate isotype-matched control IgG (eBioscience). ...
Differential Effects of Naja naja atra Venom on Immune Activity
Jian-Qun Kou … Zheng-Hong Qin, Evidence-Based Complementary and Alternative Medicine | 06/12/2014
… stained with anti-mouse CD3e-PE-cy5, CD4-PE, and CD8a-FITC antibodies (eBioscience, … splenocytes were stained with the 5-carboxyfluorescein diacetate succinimide ester (CFSE; eBioscience, San Diego, CA, USA) … incubated with anti-mouse CD4-PE or CD8a-PE antibody (eBioscience, San Diego, CA, USA). … The concentrations of IFN-γ, IL-4, and IL-17 were assayed with ELISA kits (Shanghai Hushang Biotechnology Co., Ltd., Shanghai, China or eBioscience, … plus ionomycin (1 μM; eBioscience, … and brefeldin A (BFA; 1 : 1000 dilution, eBioscience…stained with anti-mouse CD3e-APC and CD4-PE antibody (eBioscience, … washed and fixed with fixation buffer (eBioscience, … Fixed cells were washed twice with permeabilization buffer (eBioscience, … intracellular cytokine staining was carried out using FITC conjugated anti-mouse IFN-γ, IL-4, or IL-17 antibody (eBioscience, …
Disequilibrium of M1 and M2 Macrophages Correlates with the Development of Experimental Inflammatory Bowel Diseases
Wei Zhu,… Xiao-li Zhang, Immunological Investigations | 06/12/2014
... The fixation and permeabilization kit used for flow cytometry (FACS) was from eBioscience (San Diego, ... The cells were stained and incubated at room temperature for 30 min with FITC-conjugated anti-forkhead box P3 (FoxP3) (eBioscience). ...
Myeloid-derived suppressor cell function is diminished in aspirin-triggered allergic airway hyperresponsiveness in mice
Maohua Shi, … Ying Yu, Journal of Allergy and Clinical Immunology, In Press, Corrected Proof, Available online 17 June 2014
eBio excerpt not available.
Yufei Zhang … Yiwei Chu, Clinical Immunology | 06/11/2014
... Flow cytometry analysis. GL261 tumor cells (1×10 6 ) were stained with directly conjugated antibodies: anti-TLR1, -TLR2, APC-Annexin V and PE-conjugated PI, and also with appropriate isotype-matched control IgG (eBioscience). ...
Differential Effects of Naja naja atra Venom on Immune Activity
Jian-Qun Kou … Zheng-Hong Qin, Evidence-Based Complementary and Alternative Medicine | 06/12/2014
… stained with anti-mouse CD3e-PE-cy5, CD4-PE, and CD8a-FITC antibodies (eBioscience, … splenocytes were stained with the 5-carboxyfluorescein diacetate succinimide ester (CFSE; eBioscience, San Diego, CA, USA) … incubated with anti-mouse CD4-PE or CD8a-PE antibody (eBioscience, San Diego, CA, USA). … The concentrations of IFN-γ, IL-4, and IL-17 were assayed with ELISA kits (Shanghai Hushang Biotechnology Co., Ltd., Shanghai, China or eBioscience, … plus ionomycin (1 μM; eBioscience, … and brefeldin A (BFA; 1 : 1000 dilution, eBioscience…stained with anti-mouse CD3e-APC and CD4-PE antibody (eBioscience, … washed and fixed with fixation buffer (eBioscience, … Fixed cells were washed twice with permeabilization buffer (eBioscience, … intracellular cytokine staining was carried out using FITC conjugated anti-mouse IFN-γ, IL-4, or IL-17 antibody (eBioscience, …
Disequilibrium of M1 and M2 Macrophages Correlates with the Development of Experimental Inflammatory Bowel Diseases
Wei Zhu,… Xiao-li Zhang, Immunological Investigations | 06/12/2014
... The fixation and permeabilization kit used for flow cytometry (FACS) was from eBioscience (San Diego, ... The cells were stained and incubated at room temperature for 30 min with FITC-conjugated anti-forkhead box P3 (FoxP3) (eBioscience). ...
Myeloid-derived suppressor cell function is diminished in aspirin-triggered allergic airway hyperresponsiveness in mice
Maohua Shi, … Ying Yu, Journal of Allergy and Clinical Immunology, In Press, Corrected Proof, Available online 17 June 2014
eBio excerpt not available.
