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• Decreased Seizure Threshold in an Eclampsia-Like Model Induced in Pregnant Rats with Lipopolysaccharide and Pentylenetetrazol Treatments

Qian Huang, … Huishu Liu, PLOS ONE | 02/20/2014
… ELISA kits specific for tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were obtained from eBioscience (San Diego, USA) …
 
作者单位: 广州医科大学附属广州市妇女儿童医疗中心 
 

• Self-renewal and Differentiation of Muscle Satellite Cells Are Regulated by the Fas-associated Death Domain

Wei Cheng, … Zichun Hua, J. Biol. Chem., Feb 2014; 289: 5040 - 5050
...  Anti-CD34 was purchased from eBioscience. SC-derived primary myoblasts were isolated as...the anti-IgG1 isotype-specific secondary antibodies (eBioscience) were diluted in the blocking solution from the M.O... 
 
作者单位: 南京大学医药生物技术国家重点实验室； 常州南京大学高新技术研究院
 

• Toll-Like Receptor 11-Initiated Innate Immune Response in Male Mouse Germ Cells

Qiaoyuan Chen, … Daishu Han, Biol Reprod, Feb 2014; 90: 38
... The kits for TNFA (BMS 607/3), IL6 (BMS 603/2), IFNG (BMS 606), and IL12 (BMS 616) were purchased from eBioscience …   
 
作者单位: 中国医学科学院基础医学研究所/中国协和医科大学基础医学院
 

• Tim-3 signaling pathway as a novel negative mediator in lipopolysaccharide-induced endotoxic shock

Feng Wang, … Ziyong Sun, Human Immunology, In Press, Accepted Manuscript, Available online 20 February 2014
eBio excerpt not available.
 
作者单位: 华中科技大学同济医学院同济医院检验科
 

• HMGB1–LPS complex promotes transformation of osteoarthritis synovial fibroblasts to a rheumatoid arthritis synovial fibroblast-like phenotype  [open access]

Y Qin … Q Shen, Cell Death and Disease | 02/20/2014
... analyzed by flow cytometry. OASFs, tOASFs, and RASFs were stained with PE-antiTLR2 (eBioScience, San Diego, CA, USA), APC-antiTLR4 (eBioScience),  ...
 
作者单位: 第二军医大学长海医院实验诊断科
 

• Impaired Circulating CD4+LAP+ Regulatory T Cells in Patients with Acute Coronary Syndrome and Its Mechanistic Study

Zheng-Feng Zhu, … Qiu-Tang Zeng, PLOS ONE | 02/18/2014
… Cells were stimulated by exposure to soluble anti-CD3 (eBioscience, 5 µg/ml) and anti-CD28 (eBioscience, … cells were harvested and stained with anti-human LAP-APC (clone 27232, R&D Systems), anti-human CD4-FITC (R&D Systems), and anti-human GARP-PE (G14D9, eBioscience) … T cells were incubated … 100 U/ml penicillin, and 100 U/ml streptomycin at 37°C with plate-bound (5 µg/ml, eBioscience) anti-CD3 and soluble anti-CD28 (2 µg/ml, eBioscience) …
 
作者单位: 华中科技大学同济协和医院心血管病研究所生物靶向治疗教育部重点实验室
 

• Mesenchymal Stem Cells Ameliorate Th1-Induced Pre-Eclampsia-Like Symptoms in Mice via the Suppression of TNF-α Expression

Liu Liu, … Yayi Hou, PLOS ONE | 02/18/2014
… TGF-β (10 µg/ml) and IL-6 (10 µg/ml) neutralizing Ab was from Ebioscience. …  Mononuclear cells were isolated by density gradient and activated by incubation with anti-CD3 mAb (eBiosciences) at 3 µg/ml for 20 min.
 
作者单位: 南京大学医药生物技术国家重点实验室; 南京大学医学院附属鼓楼医院
 

• An unbalanced PD-L1/CD86 ratio in CD14++CD16+ monocytes is correlated with HCV viremia during chronic HCV infection

Jiajia Zheng … Fengmin Lu, Cellular and Molecular Immunology | 02/16/2014
... plasma or cultured supernatants were analyzed using ELISA kits according to the manufacturer's instructions (eBioscience, San Diego, CA, USA). The sensitivities of the ELISAs were 1.6  ...
 
作者单位: 北京大学医学部基础医学院病原生物学系
 

• T Follicular Helper Cells and Regulatory B Cells Dynamics in Systemic Lupus Erythematosus

Xue Yang, … Hejian Zou, PLOS ONE | 02/14/2014
… and 20 µg/ml brefeldin A [BFA], eBioscience, San Diego, CA) for the last 5 hours, … For some experiments, CD20+CD27− naïve B cells (eBioscience) were sorted from PBMCs of healthy donors by flow cytometry, … Tfh cells (2×106 cells/ml) were stimulated with 2 µg/ml plate-bound anti-CD3 and 2 µg/ml soluble anti-CD28 (eBioscience) … stimulated Tfh cells or 20 µg/ml anti-IL-21 neutralizing antibody (eBioscience) … IL-10 was detected in the supernatants by ELISA (eBioscience). …
 
作者单位: 复旦大学附属华山医院风湿科