X Liang ... Q Lian, Cell Death and Disease | 05/21/2015
... Cell mobility and anti-apoptosis were analyzed using transwell assay and Annexin V/PI staining (eBioscience, San Diego, CA, USA; 88-8007-74), respectively.
Jing-Yi Liu, ... Xiao-Ying Zou, Journal of Endodontics, In Press, Corrected Proof, Available online 21 May 2015
eBio excerpt not available.
Xiaoqian Wang ... Jiyan Zhang, Scientific Reports | 05/29/2015
... ELISA kits for IL-6, IL-17A, and TNF-α were from eBioscience ... cells were stained with fluorescence-conjugated antibodies against CD4 and CD25, fixed and permeabilised using a fixation/permeabilisation kit (eBioscience, San Diego, CA, USA) and stained with fluorescence-conjugated specific antibodies against IFN-γ, IL-17A, and Foxp3 in accordance with the manufacturer’s instructions.
IFN-γ differentially regulates subsets of Gr-1+CD11b+ myeloid cells in chronic inflammation
Xiaoxia Zhan, ... Minhao Wu, Molecular Immunology, In Press, Corrected Proof, Available online 26 May 2015
eBio excerpt not available.
Lin Fan, ... Zhongyi Hu, PLOS ONE | 05/27/2015
... PBMC were incubated with PPD, ESAT-6, PHA or medium alone at 37°C in 5% CO2 for 72 hours. Brefeldin A (eBioscience, BFA, final working concentration 3uL/mL) ... Samples were resuspended in 100 uL of diluted antibody against human cell-surface markers (CD4-PerCP-Cy5.5 (eBioscience,USA), ... The concentration of IFN-γ was determined using the Human IFN-γ ELISA Set (eBiosciences, USA), ... IL-17 concentrations in supernatants were quantified using the Human IL-17 ELISA Set (eBioscience, ...
Jun Wan, ... RenQuan Zhang, International Immunopharmacology, Uncorrected Proof, online 29 May 2015
eBio excerpt not available.
Jie Sun ... Na Lu, PLOS ONE | 05/29/2015
... An annexin V-FITC apoptosis detection kit, a chemiluminescent electromobility shift assay (EMSA) kit, and human IL-1β and IL-6 enzyme-linked immunosorbent assay (ELISA) kits were purchased from Bender MedSystems Co., Ltd. (Burlingame, CA, USA) ...
MicroRNA-130a Targets MAP3K12 to Modulate Diabetic Endothelial Progenitor Cell Function
Ye M. ... Lv Z., Cell Physiol Biochem | 2015;36:712-726
... Apoptotic cells were labeled with anti-annexin V (eBioscience, USA) instead of propidium iodide (PI, eBioscience) and analyzed by fluorescence activated cell sorting (FACS).
Huan Wang ... Feng Han, Cell Research | 05/22/2015
... For flow cytometry65, cells were resuspended in buffer (1% BSA in PBS) and incubated for 30 min in the dark at 4 °C with antibodies (eBioscience) initially at 1:500 to detect specific cell-surface markers, including: PE-CD18(M18/2); PE-CD11a(M17/4); PE-CD11b(M1/70); APC-Ly6G (RB6-8c5); PE-CD54 (KAT-1); FITC-CD106 (429) and APC-CD31 (390). Isotype-matched control antibodies (eBioscience) were used as negative controls ...
pH-dependent recognition of apoptotic and necrotic cells by the human dendritic cell receptor DEC205
Longxing Cao, ... Yongning He, PNAS, May 2015; 10.1073/pnas.1505924112.
...Cell Staining by Flow Cytometry. Apoptosis and necrosis was measured using Annexin V Apoptosis Detection Kit APC (eBioscience, Inc.).
Shukai Yuan, ... Zhengquan Yu, PLOS Genetics | 05/28/2015
... The following antibodies were used: Alpha6-PE (1:500, eBioscience), CD34-eFluor660 (1:100, eBioscience).
Single-Cell Transcriptome Analyses Reveal Signals to Activate Dormant Neural Stem Cells
Yuping Luo ... Siguang Li, Cell, Volume 161, Issue 5, 21 May 2015, Pages 1175-1186
eBio excerpt not available.
Xiaojun Liu, ... Yongsheng Chang, Journal of Hepatology, Accepted Manuscript, Available online 27 May 2015
eBio excerpt not available.
Spliced XBP1 promotes macrophage survival and autophagy by interacting with Beclin-1
Ping-Ge Tian, ... Qing-Hua Zhang, Biochemical and Biophysical Research Communications, In Press, Accepted Manuscript, Available online 28 May 2015
eBio excerpt not available.
