A critical role of toll-like receptor 2 (TLR2) and its’ in vivo ligands in radio-resistance
Fu Gao ... Cong Liu, Scientific Reports | 08/13/2015
... Flow cytometry staining buffer (eBioscience, San-Diego, CA, USA); ... Red blood cells were removed using RBC lysis buffer (eBioscience, San Diego, Ca).
Activation of Slit2-Robo1 signaling promotes liver fibrosis
Jianlan Chang, ... Lijing Wang, Journal of Hepatology, In Press, Accepted Manuscript, online 8 August 2015
eBio excerpt not available.
Rui Liu ... Lingyun Sun, Scientific Reports | 08/11/2015
... Tfh cells under Tfh cell-polarizing condition (3 μg/ml soluble anti-CD3/28 (eBioscience, ... 10 μg/ml anti-IL-4 antibody (eBioscience), 10 μg/ml anti-IFNγ antibody (eBioscience) ... the inhibitor of IDO enzyme activity or 10 μg/ml anti-IL-10 antibody (eBioscience) ... and IgM in the supernatant were measured by ELISA (eBioscience) ... in the presence of 2 μg/ml anti-CD3e, 1 μgCD28 (eBioscience), ... and 5 μg/ml anti-CD40 (eBioscience) ... Mice Tfh cells were stained with FITC-anti-CD4, PE-anti-CD44, APC-anti-CXCR5 and PerCP-eFluor®710-anti-PD-1 (eBioscience).
Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia
Wei Yu, ... Yizhou Zheng, Cytokine, In Press, Corrected Proof, Available online 14 August 2015
eBio excerpt not available.
Antitumor and immunomodulatory activity of a water-soluble polysaccharide from Grifola frondosa
Guang-Hua Mao, ... Xiang-Yang Wu, Carbohydrate Polymers, Accepted Manuscript, online 12 August 2015
eBio excerpt not available.
Dengfeng Xu ... Peizeng Yang, Scientific Reports | 08/13/2015
... PBMCs were stimulated with a mixture of anti-CD3 (5 μg/ml, eBioscience, SanDiego, California, USA) and anti-CD28 antibodies (1 μg/ml, eBioscience, ...
Rui-Xian Liu ... Dong-Ming Kuang, Hepatology | Accepted manuscript online: 1 AUG 2015
... or goat anti-CCL1 (eBioscience) plus mouse anti-CD68, followed by Alexa Fluor 488-or ... IgG in the supernatants from in vitro culture systems were detected using ELISA kits according to the manufacturers' instructions (eBioscience for IL-10 and IL-12p70, R&D ...
Enhancer of zeste homolog 2 silencing inhibits tumor growth and lung metastasis in osteosarcoma
Yang-Fan Lv ... Qiao-Nan Guo, Scientific Reports | 08/12/2015
... Apoptosis assay was performed with an Annexin V-APC antibody (eBioscience, Santiago, CA, UT) ... Harvested cells were washed three times in PBS and then resuspended in 300 μl binding buffer (eBioscience).
GIT2 deficiency attenuates concanavalin A-induced hepatitis in mice
Yu-E. Hao, ... Xiao-Ming Yang, FEBS Open Bio, In Press, Accepted Manuscript, Available online 11 August 2015
... All antibodies were purchased from commercial sources: CD3-phycoerythrin (PE), CD4-allophycocyanin (APC), CD4-peridinin-chlorophyll-protein complex (PerCP), CD19-PE, CD3-APC, CD3-fluorescein isothiocyanate (FITC), CD8-PE, NK1.1-APC, F4/80-PE, CD11c-PE, CD11c-APC, IL-4-PE, IFN-[1]-APC, TNF-FITC, CD69-APC, anti-CD3 Abs, and anti-CD28 Abs from eBioscience; ... Lymphocyte populations were characterized using FITC-, PE-, APC-conjugated mAbs; for Tregs, Foxp3 detection kit (eBioscience) was used ...
Lactoferrin Inhibits IL-1β-Induced Chondrocyte Apoptosis Through AKT1-Induced CREB1 Activation
Xue H. ... Mei J., Cell Physiol Biochem 2015;36:2456-2465
... Flow cytometric analysis. The apoptotic rate was evaluated using an Annexin V-FITC apoptosis detection kit (eBioscience, San Diego, CA, USA) ...
Hai Lin ... Li Hu, Scientific Reports | 08/14/2015
... concentrations of IL-4, IL-9 and IL-17 in NLF or IL-6 and IL-8 in the cell culture supernatants were assayed with specific ELISA kits (eBioscience Inc., USA).
Yufen Wei, ... Xinru Liu, Journal of Chromatography B, In Press, Accepted Manuscript, online 14 August 2015
eBio excerpt not available.
NKX6.1 functions as a metastatic suppressor through epigenetic regulation of the epithelial–mesenchymal transition [open access]
H-J Li ... Y-W Lin, Oncogene | 08/10/2015
... The following antibodies were used in IF assay: anti-E-cadherin-eFluor (eBioscience, San Diego, CA, USA), vimentin-eFluor (eBioscience) or V5-FITC (AbD Serotec).
Role of Th9 cells and Th17 cells in the pathogenesis of malignant ascites
Xian-Wen Yang, ... Shan-Yu Qin, Asian Pacific Journal of Tropical Biomedicine, In Press, Corrected Proof, Available online 7 August 2015
eBio excerpt not available.
Min Xu, ... Youli Zhang, Cancer Letters, In Press, Accepted Manuscript, Available online 11 August 2015
eBio excerpt not available.
Wei Zhang, ... Yuanji Xu, Molecular Immunology, In Press, Corrected Proof, Available online 8 August 2015
eBio excerpt not available.