Certainly! Here's a detailed breakdown of the product information for "5*蛋白还原型上样缓冲液" (5x Protein Reducing Sample Buffer):


Storage:
- Temperature: Store at -20°C for long-term storage. For short-term use, it can be kept at 4°C.
- Stability: Ensure the buffer is tightly sealed to prevent contamination and evaporation. Avoid repeated freeze-thaw cycles, which can degrade the components.


Product Description:
- This is a concentrated reducing sample buffer designed for preparing protein samples for SDS-PAGE electrophoresis. The buffer facilitates the denaturation and reduction of proteins, ensuring accurate molecular weight determination and effective separation during electrophoresis.
- Composition typically includes Tris-HCl, SDS, glycerol, bromophenol blue, and a reducing agent such as DTT (Dithiothreitol) or β-mercaptoethanol.


Key Features:
- Concentration: 5x, allowing for easy dilution to the working concentration (1x) prior to use.
- Reducing Agent: Contains a potent reducing agent to break disulfide bonds, ensuring proteins are fully denatured.
- Compatibility: Suitable for use with a wide range of protein samples and compatible with most electrophoresis systems.
- Convenience: Ready-to-use formulation simplifies sample preparation and enhances reproducibility.


What's Included:
- A vial of 5x concentrated protein reducing sample buffer.
- Detailed instructions for dilution and use.
- Safety data sheet (SDS) outlining handling and disposal protocols.


Applications:
- SDS-PAGE: Optimal for preparing samples for SDS-PAGE, ensuring effective protein separation based on molecular weight.
- Western Blotting: Enhances transfer and detection efficiency by ensuring proteins are in their reduced and denatured form.
- Protein Analysis: Suitable for various protein analysis techniques where sample denaturation is required.
- Research: Ideal for use in research laboratories studying protein structure, function, and interaction.

This comprehensive overview should provide a clear understanding of the product's usage and benefits. If you need further information or assistance, feel free to ask!