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产品描述
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TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR
Storage:
- Store the SuperMix at -20°C upon receipt.
- Avoid repeated freeze-thaw cycles to maintain product integrity and performance.
- The product is stable for at least 12 months when stored correctly.
- Ensure all components are kept on ice during use to preserve activity.
Product Description:
TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR is a comprehensive solution designed to streamline the reverse transcription process. It is optimized for the synthesis of first-strand cDNA from RNA templates. The SuperMix combines all necessary components, including reverse transcriptase, RNase inhibitor, and reaction buffer, into a single, easy-to-use format, reducing the need for multiple steps and minimizing potential errors.
Key Features:
- Convenience: All-in-one formulation simplifies the workflow, reducing preparation time and minimizing handling errors.
- Efficiency: High reverse transcription efficiency ensures robust cDNA synthesis from a broad range of RNA inputs.
- Versatility: Compatible with various types of RNA, including total RNA, mRNA, and viral RNA.
- Sensitivity: Capable of detecting low-abundance transcripts, making it suitable for challenging samples.
- Stability: Formulated for consistent performance across different experimental conditions.
What's Included:
- TransScript® Reverse Transcriptase
- RNase Inhibitor
- Optimized Reaction Buffer
- dNTP Mix
- Oligo(dT) and Random Primers
- User Manual with detailed protocols for cDNA synthesis
Applications:
- Gene Expression Analysis: Ideal for applications requiring accurate expression profiling, such as qPCR and RT-PCR.
- Cloning: Facilitates efficient cloning of cDNA for further analysis and manipulation.
- Sequencing: Prepares high-quality cDNA suitable for next-generation sequencing applications.
- Diagnostics: Useful in clinical diagnostics for detecting viral RNA or other pathogen-related RNA.
- Research: Supports a wide range of research studies, including functional genomics and biomarker discovery.
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By providing detailed information in these areas, users can better understand the capabilities and advantages of the TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR, leading to more successful experimental outcomes.
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