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Storage
- Temperature: Store at -20°C for long-term storage. Avoid repeated freeze-thaw cycles to maintain product integrity.
- Conditions: Keep in a dry environment. Protect from light to prevent degradation.
- Shelf Life: Typically stable for 6-12 months when stored properly.


Product Description
- Overview: pLV-H1-EF1a-puro is a lentiviral vector designed for gene expression studies, particularly useful for stable gene knockdown via RNA interference in mammalian cells.
- Structure: Contains the H1 promoter for driving shRNA expression and the EF1a promoter for expressing a puromycin resistance gene, allowing for selection in transduced cells.
- Size: Approximately 8.5 kb, optimized for efficient packaging and transduction.


Key Features
- Dual Promoter System: Facilitates simultaneous expression of shRNA and a selection marker.
- Puromycin Resistance: Allows for easy selection of successfully transduced cells in culture.
- High Efficiency: Lentiviral system ensures high transduction efficiency across a wide range of mammalian cell types.
- Versatility: Compatible with various cloning techniques for the insertion of shRNA sequences.


What's Included
- Vector: pLV-H1-EF1a-puro plasmid DNA, provided at a concentration suitable for immediate use or further amplification.
- Documentation: Detailed user manual with protocols for transduction, selection, and troubleshooting.
- Quality Control: Certificate of analysis confirming purity and sequence identity.


Applications
- Gene Knockdown: Effective for stable, long-term suppression of target gene expression in mammalian cells using RNA interference.
- Drug Discovery: Facilitate the study of gene function and interaction by silencing specific genes.
- Functional Genomics: Useful for high-throughput screening applications to identify gene functions and pathways.
- Cell Line Development: Engineer cell lines with stable gene expression changes for research or therapeutic purposes.
- Cancer Research: Study the effects of gene knockdown in cancer cells to identify potential therapeutic targets.

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