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产品描述
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Certainly! Let's provide a comprehensive overview of the biological product pLV-H1-EF1a-bsd by supplementing and improving its product information across various aspects:
Storage
- Temperature: Store at -20°C for long-term stability. Avoid repeated freeze-thaw cycles.
- Conditions: Ensure the product is kept in a dry environment, away from direct sunlight and moisture.
- Shelf Life: When stored properly, the product retains its functionality for up to 12 months.
Product Description
- Overview: pLV-H1-EF1a-bsd is a lentiviral vector designed for efficient gene delivery and expression in mammalian cells. It features a human H1 promoter for driving RNA interference and an EF1a promoter for robust expression of the blasticidin resistance gene.
- Components: The vector includes crucial elements such as the H1 promoter for shRNA expression, EF1a promoter for blasticidin resistance, and necessary elements for lentiviral packaging.
Key Features
- High Efficiency: Provides efficient and stable transduction of target cells, making it ideal for gene knockdown and expression studies.
- Versatile Promoters: Utilizes the H1 promoter for RNAi applications and the EF1a promoter for strong, constitutive gene expression.
- Selection Marker: Contains a blasticidin resistance gene for easy selection of successfully transduced cells.
- Compatibility: Designed to be compatible with multiple cell lines, broadening the scope of potential applications.
What's Included
- pLV-H1-EF1a-bsd Plasmid DNA: A vial containing a specific concentration of the plasmid, usually supplied in a buffer solution.
- Product Datasheet: Detailed information on vector map, sequence, and usage protocols.
- Certificate of Analysis: Verification of the product's quality and concentration.
- Technical Support: Access to expert support for troubleshooting and optimization of vector use.
Applications
- Gene Knockdown Studies: Utilized for RNA interference applications to study gene function and regulation.
- Stable Gene Expression: Ideal for creating cell lines with stable expression of target genes under the EF1a promoter.
- Drug Resistance Studies: Facilitates research in drug resistance mechanisms via blasticidin selection.
- Functional Genomics: Enables the exploration of gene networks and pathways through controlled gene expression and silencing.
By covering these aspects, you ensure users have a clear understanding of how to store, utilize, and apply pLV-H1-EF1a-bsd in various research settings.
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