Certainly! Here's a detailed outline for Fluo-3, AM (CAS 121714-22-5), a biological product used primarily for calcium imaging in cells:


Storage
- Temperature: Store at -20°C to maintain stability.
- Protection: Keep away from light to prevent degradation.
- Packaging: Ensure container is tightly closed to avoid moisture and contamination.


Product Description
Fluo-3, AM is a high-affinity fluorescent calcium indicator. It is a cell-permeant acetoxymethyl (AM) ester that allows for easy loading into live cells. Once inside the cell, esterases cleave the AM group, trapping the dye and allowing it to bind to free calcium ions. Upon binding, Fluo-3 exhibits enhanced fluorescence, making it an excellent tool for monitoring calcium dynamics within cells.


Key Features
- High Sensitivity: Exhibits significant fluorescence enhancement upon binding calcium.
- Cell Permeability: The AM ester facilitates easy entry into live cells.
- Versatility: Suitable for a wide range of applications, including microscopy and flow cytometry.
- Spectral Properties: Excitation at 488 nm and emission at around 525 nm, compatible with common fluorescence detection systems.


What's Included
- Fluo-3, AM Bulk: Typically provided in lyophilized powder or stock solution form.
- Solvent Information: Instructions for reconstitution, often with DMSO or other appropriate solvents.
- Technical Data Sheet: Detailed instructions for use, including recommended concentrations and protocols for various applications.
- Safety Data Sheet (SDS): Information on handling, hazards, and first aid measures.


Applications
- Calcium Imaging: Ideal for real-time monitoring of intracellular calcium levels in live cells.
- Flow Cytometry: Use in conjunction with other markers for multiparametric analysis.
- Confocal Microscopy: Allows for spatial resolution of calcium ion concentrations in subcellular compartments.
- Drug Screening: Assess the impact of pharmaceuticals on calcium signaling pathways.
- Neurobiology: Study calcium flux in neurons to understand signal transduction and plasticity.

By enhancing and supplementing these sections, users can better understand how to handle, utilize, and apply Fluo-3, AM in their research or clinical studies.