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产品描述
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Certainly! Here is a comprehensive product information guide for the CellTiter 96® AQueous One Solution Cell Proliferation Assay, covering various aspects:
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CellTiter 96® AQueous One Solution Cell Proliferation Assay
Product Description
The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method designed to determine the number of viable cells in proliferation or cytotoxicity assays. It utilizes a tetrazolium compound (MTS) that is bioreduced by cells into a formazan product that is soluble in tissue culture medium, allowing for easy quantification via absorbance measurements.
Storage
- Temperature: Store the CellTiter 96® AQueous One Solution at 2-8°C (refrigerator temperature).
- Conditions: Protect from light and avoid repeated freeze-thaw cycles to ensure product stability.
- Shelf Life: Refer to the expiration date on the product label for optimal performance.
Key Features
- Ease of Use: Single reagent addition for a straightforward assay setup.
- Non-Radioactive: Safe and environmentally friendly without the use of radioactive materials.
- Soluble Product: Generates a soluble formazan product for simplified absorbance reading.
- Versatility: Suitable for different cell types and adaptable to various experimental conditions.
- High Throughput: Compatible with 96-well plate formats for large-scale screening.
What's Included
- CellTiter 96® AQueous One Solution Reagent: Contains MTS and an electron coupling reagent.
- User Manual: Detailed instructions and protocols for conducting the assay.
- Safety Data Sheet: Information on safe handling and disposal procedures.
Applications
- Cell Viability Assays: Quantify viable cell numbers in various experimental setups.
- Cytotoxicity Tests: Assess the toxic effects of compounds on cell health.
- Drug Screening: Evaluate potential drug candidates for their effects on cell proliferation.
- Genetic Studies: Study the impact of gene expression changes on cell growth.
- Cancer Research: Investigate the proliferation rates of cancer cells under different conditions.
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By supplementing and improving product information in these key areas, users can better understand the capabilities and proper usage of the CellTiter 96® AQueous One Solution Cell Proliferation Assay, facilitating successful research outcomes.
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