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Product Information: Soluble Epoxide Hydrolase Inhibitor Screening Assay Kit


Storage
- Temperature: Store the kit at -20°C upon arrival.
- Stability: Ensure all components are tightly sealed to prevent moisture ingress. The kit is stable for at least 6 months from the date of receipt when stored properly.
- Handling: Avoid repeated freeze-thaw cycles. Thaw only once before use and aliquot if necessary.


Product Description
The Soluble Epoxide Hydrolase (sEH) Inhibitor Screening Assay Kit is designed for the rapid and sensitive evaluation of potential sEH inhibitors. Soluble epoxide hydrolase is an enzyme involved in the metabolism of epoxides to diols, which plays a crucial role in vascular biology and inflammation. This kit employs a fluorescence-based method to detect enzyme activity, allowing researchers to screen compounds that may inhibit sEH activity.


Key Features
- High Sensitivity: Detects low levels of sEH activity using a fluorescence-based assay.
- Rapid Screening: Facilitates quick evaluation of multiple inhibitors in a single session.
- Ease of Use: Comes with a straightforward protocol suitable for both novice and experienced researchers.
- Reliable and Reproducible: Provides consistent results across different assays.
- Comprehensive: Includes all necessary reagents for conducting assays.


What's Included
- sEH Enzyme: Purified enzyme for testing.
- Substrate: Fluorescently labeled substrate optimized for high sensitivity.
- Positive Control: Known sEH inhibitor for assay validation.
- Assay Buffer: Optimized buffer to maintain enzyme activity.
- Microplate: 96-well plate for conducting assays.
- Detailed Protocol: Step-by-step instructions for conducting the assay.


Applications
- Drug Discovery: Ideal for screening potential therapeutic compounds targeting sEH.
- Research: Useful for studying the role of sEH in various physiological and pathological processes such as inflammation and cardiovascular diseases.
- Biochemistry: Assists in the understanding of enzyme kinetics and inhibitor potency.
- Pharmacology: Facilitates the investigation of sEH inhibitors' effects on biological systems.

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