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产品描述
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Certainly! Here's a detailed product information outline for the Rat Hydroxysteroid (17-beta) Dehydrogenase 1 ELISA Kit, focusing on Storage, Product Description, Key Features, What's Included, and Applications:
Product Description:
The Rat Hydroxysteroid (17-beta) Dehydrogenase 1 ELISA Kit is designed for the quantitative detection of HSD17B1 levels in rat samples. This enzyme plays a critical role in steroid metabolism, converting estrone into the more active estradiol. The kit utilizes a sandwich ELISA format, providing accurate and reliable results for research purposes.
Key Features:
- Sensitivity: High sensitivity for detecting low concentrations of HSD17B1.
- Specificity: Specific antibodies ensure minimal cross-reactivity with other proteins.
- Quantitative Measurement: Provides precise quantification of HSD17B1 levels.
- User-Friendly Protocol: Easy-to-follow instructions suitable for both novice and experienced researchers.
- Fast and Reliable: Complete assay within a few hours, yielding reproducible results.
Storage:
- Temperature: Store the kit at 2-8°C upon arrival.
- Stability: The unopened kit is stable for up to 6 months from the date of manufacture.
- Opened Kit: Once opened, individual components should be stored according to the instructions provided in the kit manual. Typically, reconstituted standards and working solutions are best used immediately or stored at -20°C for short-term use.
What's Included:
- Pre-coated 96-well ELISA plate: Immobilized with capture antibodies specific to HSD17B1.
- Detection Antibody: Biotin-conjugated for enhanced sensitivity.
- Standard: Lyophilized recombinant HSD17B1 for creating a standard curve.
- Substrate Solution: HRP substrate for color development.
- Stop Solution: To halt the enzymatic reaction.
- Sample Diluent: For proper sample preparation and dilution.
- Wash Buffer: Concentrated solution for thorough plate washing.
- Instruction Manual: Detailed protocol for performing the assay.
Applications:
- Research Studies: Ideal for studying steroid metabolism and its regulation in various physiological and pathological states.
- Endocrinology: Investigate hormone biosynthesis and regulation in rat models.
- Drug Development: Evaluate the impact of pharmaceuticals on HSD17B1 activity.
- Biomarker Discovery: Identify potential biomarkers for diseases related to steroid hormone imbalance.
This comprehensive product information ensures that users have all necessary details to efficiently utilize the Rat Hydroxysteroid (17-beta) Dehydrogenase 1 ELISA Kit in their research endeavors.
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