Certainly! Here's a detailed product information guide for Dispase® II (neutral protease, grade II):

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Product Information for Dispase® II (Neutral Protease, Grade II)

Storage:
- Temperature: Store Dispase® II at -20°C for long-term storage to maintain its stability and enzymatic activity.
- Conditions: Keep the product in a dry, dark place to prevent moisture uptake and degradation.
- Stability: Once reconstituted, the enzyme solution should be stored at 4°C and used within 2 weeks for optimal activity. Avoid repeated freeze-thaw cycles.

Product Description:
Dispase® II is a neutral protease extracted from the culture filtrate of Bacillus polymyxa. It is designed specifically for gentle detachment of cells from tissue matrices and is widely used in the isolation of primary cells. Unlike other proteases, Dispase® II operates under mild conditions, which helps preserve cell surface antigens and receptors.

Key Features:
- Mild Activity: Operates at neutral pH, minimizing damage to cells and preserving cell surface markers.
- Versatile: Suitable for a variety of tissues including epithelial, endothelial, and fibroblast cells.
- High Purity: Grade II ensures minimal impurities, leading to consistent and reliable results.
- Optimized Concentration: Typically used at concentrations ranging from 0.5 to 2.0 mg/ml depending on the specific application and tissue type.

What's Included:
- Dispase® II Powder: Provided in a lyophilized form, ensuring stability during storage and transport.
- Instructions for Use: Detailed protocol for reconstitution and application, including recommended concentrations and incubation times.

Applications:
- Cell Isolation: Facilitates the isolation of primary cells from various tissues, including skin, liver, and lung.
- Tissue Engineering: Useful in the preparation of tissue scaffolds by removing cells while maintaining matrix structure.
- Biomedical Research: Employed in studies requiring intact cell surface proteins and receptors for accurate data interpretation.
- Regenerative Medicine: Supports the development of cell therapies by enabling the extraction and culture of specific cell types.

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Please ensure that any handling of Dispase® II follows your institution’s safety guidelines and protocols, and refer to the manufacturer's instructions for the most accurate and detailed information.