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产品描述
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Certainly! Here's a comprehensive product information outline for transfection-grade linear polyethyleneimine (MW 40000):
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Product Name:
Transfection-Grade Linear Polyethyleneimine (MW 40000)
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Storage:
- Temperature: Store at -20°C to ensure stability and longevity.
- Conditions: Keep in a tightly sealed container to prevent moisture absorption.
- Shelf Life: When stored properly, the product can be used for up to 1 year. Avoid repeated freeze-thaw cycles.
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Product Description:
Linear polyethyleneimine (PEI) with a molecular weight of 40,000 is a high-efficiency polymer used in transfection processes. This product is specifically designed to facilitate the delivery of nucleic acids into eukaryotic cells by forming complexes with DNA or RNA, thereby enhancing cellular uptake. It is characterized by its linear structure which contributes to lower cytotoxicity compared to branched PEI variants.
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Key Features:
- High Transfection Efficiency: Optimized for effective nucleic acid delivery in various cell types.
- Low Cytotoxicity: Linear structure minimizes cell damage and supports higher cell viability.
- Versatile Applications: Suitable for DNA, RNA, and siRNA transfections.
- Consistency: Manufactured to ensure batch-to-batch consistency and reliability.
- Ease of Use: Simple protocol requiring minimal optimization for most cell lines.
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What's Included:
- Polyethyleneimine Solution: Supplied in a convenient liquid form for easy handling.
- Protocol Guide: Detailed instructions for optimal transfection conditions.
- Safety Data Sheet (SDS): Comprehensive information on safe handling and disposal.
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Applications:
- Gene Expression Studies: Facilitate the introduction of plasmid DNA for transient or stable expression.
- RNA Interference: Effective in delivering siRNA for gene silencing applications.
- CRISPR/Cas9 Experiments: Assist in delivering components for genome editing.
- Protein Production: Enhance transfection efficiency for recombinant protein production in mammalian cells.
- Vaccine Development: Support nucleic acid-based vaccine research by improving transfection rates.
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This product is ideal for researchers looking to achieve high transfection efficiencies with reduced cytotoxicity, making it an invaluable tool in the fields of molecular biology, genetics, and biotechnology. Always refer to the provided protocol for specific instructions tailored to your cell type and experimental setup.
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