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BD Pharmingen™ APC Mouse Anti-Human CD8


Storage
- Temperature: Store at 2-8°C to maintain the integrity of the antibody conjugate.
- Protection from Light: The APC conjugate is light-sensitive; store in a dark place or use opaque containers to prevent degradation.
- Shelf Life: Check the expiration date on the label for effective use. Typically, the product is stable for a period of 12 months from the date of manufacture.
- Handling: Avoid repeated freeze-thaw cycles as they may denature the antibody.


Product Description
- Overview: BD Pharmingen™ APC Mouse Anti-Human CD8 is a monoclonal antibody designed for the detection of the CD8 antigen in human cells. It is conjugated with allophycocyanin (APC), a fluorochrome that facilitates flow cytometry analysis.
- Clone: The antibody is derived from the clone RPA-T8, known for its specificity and affinity for the CD8 antigen.
- Formulation: Supplied in a liquid format containing stabilizers and preservatives to ensure product longevity and performance.


Key Features
- Specificity: High specificity for human CD8, making it ideal for distinguishing CD8+ cytotoxic T cells.
- Conjugation with APC: Provides robust and clear signal in flow cytometry applications, useful for multicolor panels.
- Quality Assurance: Manufactured under stringent quality control processes to ensure consistent performance.
- Ease of Use: Ready-to-use formulation simplifies experimental setup.


What's Included
- Product Volume: Typically supplied in a vial containing sufficient antibody for 50 tests (0.5 mL).
- Instructions for Use: Detailed protocol included for optimal staining and analysis using flow cytometry.
- Certificate of Analysis: Provides batch-specific data to validate product performance.


Applications
- Flow Cytometry: Ideal for immunophenotyping studies to analyze the presence and proportion of CD8+ T cells in various samples, including peripheral blood, tissue biopsies, and cultured cells.
- Immunological Research: Useful in studies investigating immune responses, especially in contexts like cancer immunotherapy, infectious diseases, and autoimmune disorders.
- Clinical Research: Can be employed in translational research to study CD8+ T cell behavior in patient samples for diagnostic or therapeutic purposes.

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