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产品描述
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Certainly! Here’s a detailed outline of the product information for the CD4+CD25+ Regulatory T Cell Isolation Kit designed for mouse cells:
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Storage:
- Temperature: Store the kit at 2-8°C upon arrival.
- Stability: Ensure all components are kept in their original packaging to maintain stability until the expiration date.
- Avoid Freezing: Do not freeze any part of the kit as this may compromise performance.
- Light Sensitivity: Protect reagents from prolonged exposure to light.
Product Description:
The CD4+CD25+ Regulatory T Cell Isolation Kit for mice is designed to efficiently isolate CD4+CD25+ regulatory T cells from mouse splenocytes or other lymphoid tissues. Regulatory T cells play critical roles in maintaining immune tolerance and preventing autoimmune disease. This kit employs a combination of magnetic bead separation and flow cytometry-compatible antibodies to ensure high purity and viability of isolated cells.
Key Features:
- High Purity: Achieves >90% purity of CD4+CD25+ T cells using advanced magnetic separation technology.
- Ease of Use: Streamlined protocol for quick and efficient cell isolation.
- Compatibility: Designed for use with mouse samples, compatible with various downstream applications such as flow cytometry, cell culture, and functional assays.
- High Viability: Ensures high cell viability post-isolation, suitable for sensitive applications.
- Comprehensive Kit: Includes all necessary reagents and antibodies for isolation, reducing the need for additional purchases.
What's Included:
- Magnetic Beads: Optimized for the isolation of CD4+CD25+ cells.
- Antibodies: High-quality antibodies specific for CD4 and CD25 markers.
- Buffer Solutions: Pre-formulated buffers for washing and resuspension.
- Protocol: Detailed step-by-step instructions for isolation and troubleshooting tips.
- Additional Reagents: Any supplementary reagents necessary for optimal performance.
Applications:
- Immunological Research: Study of immune regulation, tolerance, and autoimmune responses.
- Cell Therapy: Potential use in developing therapeutic strategies for autoimmune diseases.
- Functional Assays: Ideal for downstream applications assessing T cell functionality.
- Gene Expression Studies: Suitable for RNA extraction and expression analysis of regulatory T cells.
- Flow Cytometry: Compatible with flow cytometry analysis for verification of cell purity and phenotype.
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This improved product information is designed to provide comprehensive details that aid researchers in effectively utilizing the kit for their experiments and applications.
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