Certainly! Here's a comprehensive guide to supplement and improve the product information for Biotin-14-dATP.

---


Biotin-14-dATP Product Information


Storage
- Temperature: Store at -20°C. Avoid repeated freeze-thaw cycles to maintain stability.
- Conditions: Keep in a dry environment and protect from light to prevent degradation.
- Shelf Life: Typically stable for 12 months when stored correctly.


Product Description
Biotin-14-dATP is a modified nucleotide used for labeling DNA during polymerase chain reactions (PCR) and other biochemical applications. The biotin moiety allows for easy detection and purification, as it binds specifically to streptavidin and avidin proteins. This product is ideal for applications requiring non-radioactive labeling.


Key Features
- High Sensitivity: Provides strong signal in detection assays due to efficient incorporation into DNA.
- Versatile Labeling: Suitable for various applications, including PCR, DNA sequencing, and hybridization.
- Non-Radioactive: Safe alternative to radioactive labeling methods.
- Easy Detection: Biotin-streptavidin interaction facilitates straightforward detection processes.
- Compatibility: Works well with most DNA polymerases and standard protocols.


What's Included
- Biotin-14-dATP Solution: Supplied in a buffered aqueous solution for direct use in experiments.
- Concentration: Typically provided at a concentration of 1 mM for optimal incorporation.
- Datasheet: Detailed instructions and guidelines for use.


Applications
- DNA Labeling: Ideal for labeling DNA fragments in PCR, sequencing, and hybridization assays.
- Blotting Techniques: Useful in Southern and Northern blot assays for detecting nucleic acids.
- Affinity Purification: Facilitates purification of biotin-labeled DNA using streptavidin-coated surfaces.
- In Situ Hybridization: Suitable for detecting specific DNA sequences in fixed cells or tissues.
- Signal Amplification: Enhances sensitivity in assays where detection of low-abundance targets is necessary.

---

By providing detailed information on storage conditions, product description, key features, what's included, and applications, users can better understand how to utilize Biotin-14-dATP effectively in their research and diagnostic assays.