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Human Plasminogen Activator Inhibitor-1 (PAI-1) Activity Assay Kit


Storage
- Temperature: Store the kit at -20°C upon receipt.
- Stability: Ensure all components are kept tightly sealed and avoid repeated freeze-thaw cycles to maintain activity and performance integrity.
- Shelf Life: When stored properly, components are stable for up to 6 months from the date of receipt.


Product Description
The Human PAI-1 Activity Assay Kit is designed to measure PAI-1 activity in biological samples. PAI-1 is a crucial regulator of fibrinolysis, inhibiting tissue plasminogen activator (tPA) and urokinase (uPA). This assay provides a valuable tool for research into coagulation, cardiovascular diseases, cancer, and fibrosis. It utilizes a colorimetric or fluorometric detection method for quantifying active PAI-1 in plasma, serum, or cell culture supernatants.


Key Features
- Sensitivity: High sensitivity for detecting low levels of PAI-1 activity.
- Specificity: Specific measurement of active PAI-1, minimizing cross-reactivity.
- Ease of Use: User-friendly protocol with clear instructions for reliable and reproducible results.
- Versatility: Suitable for various sample types including plasma, serum, and cell culture media.
- Rapid Results: Obtain results in as little as 2-3 hours.


What's Included
- PAI-1 Standard: For generating a standard curve.
- Assay Buffer: Optimized for maximum activity detection.
- Detection Reagent: For colorimetric or fluorometric reading.
- Positive Control: Ensures assay functionality.
- Microplate: 96-well format for easy handling and scalability.
- Detailed Protocol: Step-by-step instructions for conducting the assay.


Applications
- Research: Investigate the role of PAI-1 in thrombosis, cardiovascular diseases, and cancer progression.
- Diagnostics: Potential use in developing diagnostic tests for conditions associated with altered fibrinolysis.
- Drug Development: Evaluate the efficacy of therapeutic agents targeting PAI-1 activity.
- Biomarker Discovery: Identify PAI-1 as a biomarker for various pathological conditions.

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This structured format provides comprehensive and clear information to potential users of the assay kit, highlighting its utility in research and potential applications in diagnostics and drug development.