Certainly! Here's a comprehensive overview of the product information for Alexa Fluor® 647 Mouse IgM, κ Isotype Control:


Storage
- Temperature: Store at 2-8°C. Protect from light.
- Stability: Do not freeze. Ensure that the product is tightly sealed to prevent contamination.
- Shelf Life: Refer to the manufacturer's guidelines for specific shelf life details, typically around 6 months to a year.


Product Description
Alexa Fluor® 647 Mouse IgM, κ Isotype Control is a fluorescently labeled immunoglobulin used as a negative control in various immunological assays. It is conjugated with Alexa Fluor® 647 dye, which emits in the far-red spectrum, making it suitable for flow cytometry and fluorescence microscopy applications. This product is designed to help distinguish between specific and nonspecific binding, ensuring accurate interpretation of experimental results.


Key Features
- Fluorophore: Alexa Fluor® 647, known for high brightness and photostability.
- Emission/Excitation: Excitation at 650 nm and emission at 668 nm.
- Isotype: Mouse IgM, κ, suitable as an isotype control for studies involving mouse antibodies.
- Specificity: Non-specific binding control to validate experimental specificity.


What's Included
- Alexa Fluor® 647 Mouse IgM, κ Isotype Control: Supplied in a liquid format.
- Concentration: Typically provided at 0.2 mg/mL or as specified on the product datasheet.
- Volume: Available in various sizes (e.g., 100 µL, 500 µL) depending on the supplier.


Applications
- Flow Cytometry: Used to assess the background fluorescence and ensure specific staining in cell surface or intracellular staining assays.
- Fluorescence Microscopy: Provides a control for non-specific antibody binding in immunofluorescence studies.
- Western Blotting: Can be used as a control in experiments requiring isotype verification.
- Immunohistochemistry: Provides a baseline for non-specific staining in tissue sections.


Additional Recommendations
- Handling: Handle with care, using appropriate personal protective equipment (PPE) to avoid exposure to the fluorescent dye.
- Usage: Follow the recommended dilution and staining protocols provided by the manufacturer for optimal results.
- Quality Control: Verify product integrity by checking for any turbidity or precipitation before use.

This information should provide a comprehensive understanding of the Alexa Fluor® 647 Mouse IgM, κ Isotype Control for effective use in your research applications. Always refer to the manufacturer's datasheet for specific details and any updates.