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产品描述
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Certainly! Here's a detailed supplement and improvement of the product information for Alexa Fluor® 488 Mouse IgG2b, κ Isotype Control:
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Product Name:
Alexa Fluor® 488 Mouse IgG2b, κ Isotype Control
Storage:
- Temperature: Store at 2-8°C.
- Conditions: Protect from light to prevent photobleaching of the fluorescent dye.
- Stability: Avoid repeated freeze-thaw cycles. If needed, aliquot the product upon first thaw to prevent degradation.
- Shelf Life: Check the manufacturer’s label for the expiration date, typically stable for 6-12 months when stored correctly.
Product Description:
Alexa Fluor® 488 Mouse IgG2b, κ isotype control is a fluorescently labeled immunoglobulin that serves as an essential control in flow cytometry, immunofluorescence, and other immunoassays. It is conjugated to Alexa Fluor® 488, a bright green fluorescent dye with high photostability and quantum yield, making it ideal for detecting and quantifying cellular markers.
Key Features:
- Fluorescent Dye: Alexa Fluor® 488 provides strong fluorescence with minimal photobleaching.
- Excitation/Emission: Optimal excitation at 495 nm and emission at 519 nm.
- Specificity: Non-specific binding control for mouse IgG2b antibodies.
- Versatility: Suitable for various applications such as flow cytometry, immunofluorescence, and confocal microscopy.
- Quality: High purity and low endotoxin levels ensure reliable experimental results.
What's Included:
- Product: Alexa Fluor® 488 Mouse IgG2b, κ Isotype Control antibody.
- Volume: Typically provided in a 100 µL vial, but check packaging for specific quantity.
- Documentation: Includes a datasheet with experimental protocols, storage instructions, and safety data.
Applications:
- Flow Cytometry: Use as a negative control to assess non-specific binding of primary antibodies and optimize gating strategies.
- Immunofluorescence: Ideal for use as a control to differentiate specific staining from background fluorescence.
- Confocal Microscopy: Provides a reliable control for fluorescence imaging experiments, confirming antibody specificity.
- Western Blot: Although primarily used in fluorescence-based assays, it can occasionally serve as a control in blotting applications to validate antibody specificity.
Additional Notes:
- Always perform a pilot experiment to determine the optimal concentration for your specific application.
- Ensure compatibility with your instrument settings, especially concerning the excitation and emission parameters of Alexa Fluor® 488.
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This comprehensive product information is designed to aid researchers in effectively utilizing the Alexa Fluor® 488 Mouse IgG2b, κ Isotype Control in their experimental workflows.
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