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Product Information for Hot Start Cesium Klentaq AC


Storage
- Temperature Requirements: Store at -20°C for optimal stability. Avoid repeated freeze-thaw cycles to maintain enzyme activity.
- Handling Instructions: Keep the enzyme on ice when setting up reactions. Ensure all components are fully thawed and mixed before use.
- Shelf Life: Stable for up to 12 months when stored correctly. For long-term storage, aliquot and freeze to prevent degradation.


Product Description
Hot Start Cesium Klentaq AC is an advanced thermostable DNA polymerase designed for PCR applications requiring high specificity and reduced non-specific amplification. Enhanced with cesium ions, this enzyme allows for rapid and reliable amplification even in challenging templates.


Key Features
- Hot Start Activation: Ensures that enzyme activity is inhibited at room temperature and activated at elevated temperatures, reducing non-specific amplification.
- Cesium Enhanced: Improves enzyme fidelity and performance across various PCR conditions.
- High Specificity: Minimizes primer-dimer formation and non-specific amplification.
- Robust Performance: Capable of amplifying complex and GC-rich templates.
- Thermostability: Maintains activity at high temperatures, suitable for long PCR runs.


What's Included
- Hot Start Cesium Klentaq AC Enzyme: Supplied in a concentrated format for flexible reaction setup.
- 10X Reaction Buffer: Optimized for maximal enzyme performance and stability.
- MgCl2 Solution: Provided separately to allow for concentration adjustments based on template requirements.
- User Manual: Comprehensive guide for setup, reaction optimization, and troubleshooting.


Applications
- Standard PCR: Suitable for routine DNA amplification tasks with high specificity.
- High-Fidelity PCR: Ideal for applications requiring precise replication, such as cloning and sequencing.
- Complex Template Amplification: Effective in amplifying difficult templates, including those with high GC content.
- Quantitative PCR (qPCR): Can be adapted for use in quantitative applications requiring accurate amplification.
- Mutagenesis Studies: Utilized in protocols that require precise and controlled amplification for mutagenesis.

This information provides a comprehensive overview of Hot Start Cesium Klentaq AC, addressing essential aspects that users need to know for successful application and optimal results in their experiments.