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Storage
- Temperature: Store Puromycin solution at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
- Protection: Keep the product protected from light to maintain its stability and effectiveness.
- Shelf Life: When stored properly, the solution remains stable for up to 12 months. Once thawed, it should be used within a week if stored at 4°C.


Product Description
Puromycin is an aminonucleoside antibiotic derived from Streptomyces alboniger. It inhibits protein synthesis by causing premature chain termination during translation. This makes it a useful tool in molecular biology for selecting cells that express specific resistance genes.


Key Features
- Potency: High efficacy in inhibiting protein synthesis, leading to rapid selection of resistant cells.
- Purity: The solution is highly purified to ensure consistency in experimental results.
- Convenience: Ready-to-use solution that can be easily integrated into various protocols.
- Versatility: Suitable for use in a wide range of cell types, including mammalian and bacterial cells.


What's Included
- Puromycin Solution: 10 mg/ml in a sterile aqueous solution.
- Certificate of Analysis: Ensures product quality and provides detailed information about the batch purity and concentration.
- Usage Instructions: Guidelines for safe handling and effective application.


Applications
- Cell Culture: Used to select for cells that have been transfected with a puromycin resistance gene, allowing for the maintenance of stable cell lines.
- Genetic Engineering: Facilitates the creation of knockout and knock-in models by selecting for successfully modified cells.
- Research: Essential in studies involving protein synthesis, gene expression, and antibiotic resistance mechanisms.
- Drug Development: Aids in the screening of compounds affecting translational machinery.


Handling Instructions
- Safety Precautions: Handle with care, using personal protective equipment such as gloves and eye protection.
- Disposal: Follow institutional guidelines for the disposal of antibiotics and chemicals to prevent environmental contamination.

By ensuring this comprehensive product information, users can effectively utilize Puromycin in their research and applications, while maintaining safety and product integrity.