Certainly! Here's a comprehensive product information supplement for Klenow (3′→ 5′ exo-) (High Concentration):


Product Information for Klenow (3′→ 5′ exo-) (High Concentration)


Storage
- Temperature: Store at -20°C.
- Conditions: Keep the product away from direct sunlight and avoid repeated freeze-thaw cycles to maintain enzyme activity.
- Shelf Life: Refer to the expiration date on the packaging for optimal use.


Product Description
Klenow (3′→ 5′ exo-) (High Concentration) is a modified form of the DNA polymerase I enzyme from *Escherichia coli*, specifically engineered to lack the 3′→ 5′ exonuclease activity. This form of Klenow fragment is utilized for DNA synthesis applications that require a polymerase with high fidelity and processivity without the proofreading function.


Key Features
- High Concentration: Offers 10,000 Units at 50,000 U/ml, suitable for demanding applications requiring substantial enzyme activity.
- Exonuclease Deficient: Lacks 3′→ 5′ exonuclease activity, reducing degradation of DNA ends during synthesis.
- High Fidelity: Ensures accurate DNA replication, ideal for applications where precision is critical.
- Robust Performance: Designed to perform efficiently under various buffer conditions, ensuring consistent results.


What's Included
- Vial of Klenow (3′→ 5′ exo-) (High Concentration): 10,000 Units enzyme in a 50,000 U/ml solution.
- Storage Buffer: Contains necessary stabilizers and components to maintain enzyme activity.
- Protocol Document: Detailed usage instructions and troubleshooting tips.


Applications
- DNA Labeling: Suitable for random primed labeling and end-labeling of DNA fragments.
- Second Strand Synthesis: Used in cDNA synthesis to generate double-stranded DNA from single-stranded templates.
- Blunt-Ending DNA Fragments: Ideal for converting overhangs created by restriction enzymes to blunt ends for cloning.
- DNA Repair: Can be used in repair processes where high fidelity synthesis is required without exonuclease activity.

For further inquiries or technical support, please refer to the contact information provided with your purchase or visit our website. Ensure to follow all safety guidelines and protocols when handling biological products.