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产品描述
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Certainly! Here's an enhanced product information guide for the ELISpot Plus: Porcine IFN-γ (ALP):
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Product Name: ELISpot Plus: Porcine IFN-γ (ALP)
Storage:
- Temperature: Store at 2-8°C. Do not freeze.
- Shelf Life: Typically stable for up to 12 months from the date of manufacture when stored correctly.
- Handling: Protect from light and moisture. Ensure reagents are capped tightly after use.
Product Description:
ELISpot Plus: Porcine IFN-γ (ALP) kit is designed for the detection and quantification of Porcine Interferon Gamma (IFN-γ) at the single-cell level. This assay utilizes enzyme-linked immunospot technology, employing alkaline phosphatase (ALP) as the detection enzyme, to identify cells secreting IFN-γ. The kit is optimized for high sensitivity and specificity, enabling researchers to analyze immune responses in porcine models accurately.
Key Features:
- High Sensitivity: Capable of detecting low frequency IFN-γ secreting cells.
- Specificity: Optimized antibodies ensure precise detection of porcine IFN-γ.
- Versatility: Suitable for various sample types, including PBMCs and splenocytes.
- Ease of Use: Comprehensive kit with detailed protocol for straightforward implementation.
- Quantitative Results: Enables enumeration of cytokine-secreting cells, providing valuable insights into immune function.
What's Included:
- Pre-coated ELISpot Plates: Plates pre-coated with capture antibodies specific for porcine IFN-γ.
- Detection Antibody: Biotinylated anti-porcine IFN-γ antibody for binding to secreted cytokines.
- ALP-Conjugated Streptavidin: Facilitates colorimetric detection of spots.
- Substrate Reagents: For development of colorimetric signal.
- Wash Buffer: Concentrated wash solution for plate washing.
- Blocking Buffer: For minimizing background noise.
- Detailed Protocol: Step-by-step instructions to ensure accurate results.
- Kit Components: Sufficient materials for multiple assays (typically 5-10 plates).
Applications:
- Immunological Research: Analysis of cellular immune responses in porcine models.
- Vaccine Development: Evaluation of vaccine efficacy through measurement of IFN-γ secretion.
- Disease Studies: Investigation of immune responses in infectious diseases affecting pigs.
- Veterinary Research: Enhancing understanding of pig immune system for health management.
- Basic Research: Fundamental studies in cytokine biology and immune regulation in swine.
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These enhancements should provide a comprehensive overview of the product, aiding researchers in understanding its utility and application in their work.
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