|
产品描述
|
Certainly! Here's a detailed and comprehensive product information sheet for the GLS Antibody (C-term):
---
GLS Antibody (C-term) - Product Information Sheet
Storage:
- Store the GLS Antibody at -20°C for long-term preservation.
- Upon receipt, aliquot the antibody to avoid repeated freeze-thaw cycles.
- For short-term use, the antibody can be stored at 4°C for up to one month.
Product Description:
The GLS Antibody (C-term) is a polyclonal antibody designed to target the C-terminal region of the Glutaminase (GLS) enzyme. GLS is a key enzyme responsible for the conversion of glutamine to glutamate, playing a crucial role in cellular metabolism, especially in cancer and neurological disorders. This antibody is produced in rabbits and purified using affinity chromatography, ensuring specificity and high binding affinity.
Key Features:
- Specificity: High specificity for the C-terminal region of GLS, minimizing cross-reactivity with other proteins.
- Sensitivity: Suitable for detecting endogenous levels of GLS in various sample types.
- Quality: Validated for use in multiple applications, ensuring reliability and consistency in results.
- Format: Available in liquid form, ready for immediate use in assays.
What's Included:
- 100 µL of GLS Antibody (C-term) at a concentration of 1 mg/mL.
- A detailed protocol for antibody usage in various applications.
- Certificate of Analysis providing batch-specific information.
Applications:
- Western Blotting: Ideal for detecting GLS protein levels in cell lysates and tissue samples.
- Immunohistochemistry (IHC): Suitable for staining formalin-fixed, paraffin-embedded tissue sections to visualize GLS expression.
- Immunoprecipitation: Can be used to precipitate GLS from complex protein mixtures for subsequent analysis.
- ELISA: Useful for quantifying GLS levels in biological samples in sandwich or competitive ELISA formats.
- Research Areas: Cancer metabolism, neurobiology, and metabolic disorders.
Usage Notes:
- Optimal dilution should be determined by the user, typically ranging from 1:500 to 1:2000 for Western Blot and 1:100 to 1:500 for IHC.
- Ensure proper controls are used to validate the specificity and effectiveness of the antibody in your specific assay conditions.
---
This product information sheet provides a comprehensive overview of the GLS Antibody (C-term) to assist researchers in planning their experiments and maximizing the utility of the antibody in various applications.
|