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产品描述
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Product Information: GAR CellTag™ 700 Stain ICW Kit II
Storage:
- Store the kit at 2-8°C (36-46°F) to maintain its efficacy.
- Avoid exposure to light. Keep the kit components in their original packaging to protect them from light damage.
- Ensure all reagents are tightly sealed to prevent contamination and evaporation.
Product Description:
- The GAR CellTag™ 700 Stain ICW Kit II is designed for in-cell western (ICW) assays, providing a robust method for quantifying protein levels directly in cultured cells.
- This kit contains a fluorescent dye that specifically tags proteins, enabling precise detection and quantification through fluorescence imaging systems.
- Ideal for researchers needing reliable results in protein expression studies, signal transduction analysis, and other cell-based assays.
Key Features:
- High Sensitivity: The fluorescent stain offers high sensitivity for detecting low-abundance proteins.
- Versatile Compatibility: Compatible with various cell types and imaging platforms, including scanners and microscopes with 700 nm excitation.
- Stable Performance: The dye provides stable signals with minimal photobleaching, ensuring consistent results over time.
- User-Friendly Protocol: Includes easy-to-follow instructions for quick and efficient assay setup.
- Multiplexing Capability: Allows simultaneous detection of multiple targets when used with complementary kits.
What's Included:
- Fluorescent dye for protein tagging.
- Blocking buffer to minimize non-specific binding.
- Wash buffer to maintain cell integrity during the assay.
- Detailed protocol booklet outlining usage instructions, tips, and best practices.
- Quality control certifications ensuring product reliability and consistency.
Applications:
- Protein Quantification: Suitable for quantifying protein expression levels in various cell lines.
- Signal Pathway Analysis: Useful for studying cellular signaling pathways by monitoring protein phosphorylation and other modifications.
- Drug Screening: An excellent tool for assessing the impact of pharmaceutical compounds on protein expression.
- Cancer Research: Supports the investigation of oncogenes and tumor suppressor proteins in cancer cell models.
- Cell Biology Studies: Facilitates research in cell growth, differentiation, and apoptosis through protein detection.
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This structured information aims to provide comprehensive insights into the GAR CellTag™ 700 Stain ICW Kit II, enhancing understanding and usability for researchers and laboratory professionals.
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