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产品描述
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Certainly! Here’s a comprehensive guide to supplement and improve the product information for Recombinant SEAP Protein:
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Product Name: Recombinant SEAP Protein
Product Description:
Recombinant SEAP (Secreted Alkaline Phosphatase) Protein is a highly purified enzyme derived from human placental alkaline phosphatase and expressed in a recombinant host. It is commonly used as a reporter gene product in various biological and biochemical assays due to its stability and ease of detection. SEAP can be secreted into the culture medium, allowing for non-invasive monitoring of gene expression.
Key Features:
- High Purity: The protein is purified to ensure optimal activity and consistency across experiments.
- Stability: Recombinant SEAP is stable across a wide range of temperatures and pH levels, making it ideal for various assay conditions.
- Versatility: Can be used in multiple applications, including gene expression studies, cell signaling assays, and drug screening.
- Ease of Detection: SEAP activity can be easily measured using colorimetric or chemiluminescent substrates.
Storage:
- Temperature: Store at -20°C for long-term storage. For short-term use, it can be kept at 4°C.
- Avoid Repeated Freeze-Thaw Cycles: To maintain protein integrity, aliquot the protein into smaller volumes upon first thawing.
- Shelf Life: Typically stable for up to 12 months when stored properly.
What's Included:
- Recombinant SEAP Protein: Provided in a buffered solution for immediate use.
- User Manual: Detailed instructions for handling, storage, and assay protocols.
- Certificate of Analysis: Includes purity, concentration, and activity information.
- Optional Substrates: Depending on the package, colorimetric or chemiluminescent substrates for SEAP activity detection may be included.
Applications:
- Reporter Gene Assays: Utilize SEAP as a reporter for monitoring gene expression in mammalian cells.
- Cell Signaling Studies: Investigate signaling pathways by linking SEAP expression to pathway activation.
- Drug Screening: Assess the impact of compounds on gene expression and enzyme activity.
- Biomarker Development: Use SEAP as a biomarker in various research and diagnostic applications.
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This comprehensive guide ensures that users have all the necessary information to utilize Recombinant SEAP Protein effectively in their research or applications.
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