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产品描述
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Product Name: Waf1/Cip1/CDKN1A p21 (F-5): m-IgG2b BP-HRP Kit
Storage:
- Store at -20°C for long-term storage.
- Once opened, the kit components should be stored at 4°C.
- Avoid repeated freeze-thaw cycles to maintain product integrity.
- Protect from light and moisture.
Product Description:
- This kit includes monoclonal antibody IgG2b specific for Waf1/Cip1/CDKN1A p21, conjugated with HRP (Horseradish Peroxidase) for enhanced detection.
- The p21 protein is a cyclin-dependent kinase inhibitor involved in cell cycle regulation, making it a crucial target for cancer research.
- The antibody is derived from mouse hybridoma cells and has been purified for optimal performance in various assays.
Key Features:
- High Specificity: The monoclonal antibody exhibits high specificity for the p21 protein, ensuring accurate detection and minimal cross-reactivity.
- HRP Conjugation: Facilitates sensitive and rapid enzymatic detection through chromogenic or chemiluminescent substrates.
- Versatile: Suitable for use in multiple applications, including Western blotting, ELISA, and immunohistochemistry.
- Quality Controlled: Each batch undergoes rigorous testing to ensure consistent performance and reliability.
What's Included:
- Primary Antibody: m-IgG2b anti-p21 antibody conjugated to HRP.
- Blocking Buffer: To minimize non-specific binding and enhance signal quality.
- Dilution Buffer: For optimal antibody dilution and application.
- Detection Substrate: Ready-to-use substrate for HRP to visualize protein-antibody complexes.
- Instruction Manual: Detailed guide for protocol optimization and troubleshooting.
Applications:
- Western Blotting: For detection and quantification of p21 protein levels in various sample types.
- ELISA: Suitable for quantitative measurement of p21 in cell lysates and tissue extracts.
- Immunohistochemistry: Allows localization of p21 expression in tissue sections, aiding in histopathological studies.
- Research: Ideal for studies focused on cell cycle regulation, cancer research, and apoptosis mechanisms.
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