Certainly! Here is a more detailed product information breakdown for the Polyclonal Rabbit anti-Human ADRA2A Antibody, covering the aspects you mentioned:


Storage
- Temperature: Store at -20°C for long-term stability. Avoid repeated freeze-thaw cycles to maintain antibody integrity.
- Stability: The antibody remains stable for up to 12 months when stored properly.
- Handling: Before use, thaw the antibody at room temperature and gently mix. After use, aliquot and store any remaining antibody to avoid contamination.


Product Description
- Species Reactivity: Human.
- Host Species: Rabbit.
- Clonality: Polyclonal.
- Purification: Affinity purified for high specificity and reduced background.
- Concentration: Typically supplied at 1 mg/mL, but verify the exact concentration on the product label.
- Target: Alpha-2A adrenergic receptor (ADRA2A), involved in neurotransmitter release and blood vessel constriction.


Key Features
- High Specificity: Designed to specifically bind the human ADRA2A protein, minimizing non-specific binding.
- Versatility: Suitable for use in various applications including immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting (WB).
- Quality Assurance: Each batch is thoroughly tested for performance consistency and reliability.


What's Included
- Antibody Vial: Contains the polyclonal rabbit anti-human ADRA2A antibody.
- Data Sheet: Provides detailed information on optimal working concentrations, protocols, and validation results.
- Certificate of Analysis: Confirms the quality and efficacy of the antibody batch.


Applications
- Immunohistochemistry (IHC): Useful for detecting ADRA2A expression in tissue sections. Recommended dilution: 1:100 – 1:500.
- Immunofluorescence (IF): Suitable for visualizing ADRA2A in cultured cells using fluorescently labeled secondary antibodies. Recommended dilution: 1:50 – 1:200.
- Western Blot (WB): Effective for detecting ADRA2A protein levels in cell lysates. Recommended dilution: 1:500 – 1:2000.
- Research Uses: Ideal for studies on neurotransmitter regulation, cardiovascular research, and investigations into mental health disorders.


Additional Notes
- Protocol Optimization: For best results, optimize antibody concentration and incubation times specific to your experimental conditions.
- Safety Precautions: Handle reagents according to safety guidelines and dispose of waste in accordance with local regulations.

This comprehensive information should aid in the effective use and understanding of the Polyclonal Rabbit anti-Human ADRA2A Antibody for your research needs.