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产品描述
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Certainly! Here's a detailed supplement and improvement for the product information of the Annexin V-APC/7-AAD Apoptosis Kit.
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Storage:
- Store the kit at 2-8°C, protected from light.
- Avoid freezing the kit components as it may affect the assay performance.
- The kit components are stable for at least 6 months when stored under these conditions.
Product Description:
The Annexin V-APC/7-AAD Apoptosis Kit is designed for the detection of early and late apoptotic cells using flow cytometry. Annexin V is conjugated to Allophycocyanin (APC) and binds specifically to phosphatidylserine residues, which translocate to the outer leaflet of the plasma membrane during apoptosis. 7-Aminoactinomycin D (7-AAD) is a nucleic acid dye that penetrates and stains DNA of cells with compromised membrane integrity, allowing differentiation between early apoptotic, late apoptotic, and necrotic cells.
Key Features:
- High Sensitivity: Detects early apoptosis by binding to phosphatidylserine with high specificity.
- Dual Staining: Differentiates between live, early apoptotic, and late apoptotic/necrotic cells.
- Fast and Reliable: Provides results in under 2 hours, suitable for high-throughput analysis.
- Flow Cytometry Compatible: Optimized for use with standard flow cytometers.
What's Included:
- Annexin V-APC Reagent: Fluorescently labeled Annexin V for detecting apoptotic cells.
- 7-AAD Solution: DNA dye for identifying necrotic or late apoptotic cells.
- Binding Buffer: Facilitates the binding of Annexin V to phosphatidylserine.
- Protocol Guide: Detailed instructions for optimal usage and results interpretation.
Applications:
- Apoptosis Research: Ideal for studying programmed cell death in various types of cells.
- Cancer Research: Analyze the effects of anti-cancer drugs on inducing apoptosis in tumor cells.
- Toxicological Studies: Assess the cytotoxic effects of new compounds on cell viability.
- Immunology: Investigate the role of apoptosis in immune cell regulation and response.
This kit is a powerful tool for researchers looking to understand cellular mechanisms at the molecular level, providing insights into the processes of apoptosis and cell death critical for advancements in biological and medical research.
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This enhanced product information should provide comprehensive details to support users in maximizing the utility of the Annexin V-APC/7-AAD Apoptosis Kit.
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