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产品描述
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Certainly! Here's a comprehensive overview of the biological product PCNA (PT0224R) PT™ Rabbit mAb, supplemented with detailed information on various aspects:
Product Description:
PCNA (Proliferating Cell Nuclear Antigen) is a protein that plays a crucial role in DNA replication and repair. It acts as a processivity factor for DNA polymerase in eukaryotic cells, ensuring efficient DNA synthesis. The PT™ Rabbit Monoclonal Antibody (mAb) against PCNA is designed for high specificity and sensitivity, making it ideal for various applications in cell biology research.
Key Features:
- High Specificity and Sensitivity: The PT™ Rabbit mAb is developed to recognize PCNA with minimal cross-reactivity, providing accurate results in assays.
- Monoclonal Antibody: Offers consistent and reproducible results with uniform affinity.
- Validated Applications: Suitable for use in various techniques, including Western blotting, immunohistochemistry, and ELISA.
- Quality Assurance: Each batch undergoes rigorous quality control to ensure optimal performance.
Storage:
- Recommended Conditions: Store at -20°C for long-term preservation. Avoid repeated freeze-thaw cycles to maintain antibody integrity.
- Stability: The antibody remains stable for up to 12 months when stored at the recommended temperature.
- Handling: Thaw on ice before use and aliquot to prevent degradation from multiple freeze-thaw cycles.
What's Included:
- PCNA (PT0224R) PT™ Rabbit mAb: Supplied in a concentration suitable for immediate use or dilution as required for specific applications.
- Certificate of Analysis: Provides detailed information on antibody specificity, sensitivity, and batch consistency.
- Product Datasheet: Includes technical data, experimental protocols, and troubleshooting tips.
Applications:
- Western Blotting: Detect PCNA protein levels in cell and tissue lysates, aiding in studies of cell proliferation and DNA repair mechanisms.
- Immunohistochemistry (IHC): Localize PCNA in tissue sections to assess cell proliferation rates in various biological contexts, including cancer research.
- Enzyme-Linked Immunosorbent Assay (ELISA): Quantify PCNA expression in different samples, useful for high-throughput screening.
- Flow Cytometry: Analyze PCNA expression at the single-cell level to study cell cycle progression.
- Research Areas: Applicable in cancer research, cell cycle studies, DNA replication, and repair investigations.
By providing comprehensive information on storage, product description, key features, inclusions, and applications, researchers can effectively utilize the PCNA (PT0224R) PT™ Rabbit mAb in their studies.
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