Certainly! Here's a comprehensive product information guide for Collagenase Type V:

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Product Name: Collagenase Type V

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Product Description:

Collagenase Type V is a highly purified enzyme derived from Clostridium histolyticum. It is specifically designed to degrade collagen, a major component of the extracellular matrix in connective tissues. This enzyme plays a crucial role in tissue dissociation and is widely used in various biomedical and research applications for the isolation of cells from tissues.

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Key Features:

- High Purity: Collagenase Type V is purified to ensure minimal contamination with other enzymatic activities, providing reliable and consistent results.
- Optimal Activity: Exhibits high enzymatic activity at physiological pH and temperature, making it suitable for a wide range of applications.
- Versatile Use: Effective for isolating cells from a variety of tissues, including pancreas, liver, adipose, and dermal tissues.
- Controlled Digestion: Allows for controlled digestion of collagen, minimizing damage to cells and preserving their viability and functionality.

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Storage:

- Temperature: Store at -20°C to maintain stability and activity over time.
- Conditions: Keep the enzyme in a dry, moisture-free environment. Avoid repeated freeze-thaw cycles to preserve enzymatic activity.
- Shelf Life: When stored properly, Collagenase Type V remains active for up to 12 months from the date of manufacture.

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What's Included:

- Collagenase Type V Enzyme: Supplied in lyophilized form for stability and ease of use.
- Certificate of Analysis: Provides detailed information on enzyme activity, purity, and lot specifications.
- Usage Instructions: Comprehensive guidelines for reconstitution and application in various experimental protocols.

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Applications:

- Tissue Dissociation: Efficiently breaks down collagen to facilitate the isolation of single cells or cell clusters from tissues.
- Cell Isolation: Used in the preparation of primary cells from organs such as the pancreas, liver, and adipose tissue for research and therapeutic purposes.
- Histological Studies: Assists in studying tissue architecture and cellular interactions by enabling the breakdown of structural barriers.
- Biomedical Research: Supports studies in wound healing, fibrosis, cancer research, and regenerative medicine by enabling the manipulation of the extracellular matrix.

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By enhancing the product information across these aspects, users can gain a well-rounded understanding of Collagenase Type V's capabilities and applications, ensuring effective use in their research and experimental endeavors.