Certainly! Here's a comprehensive overview of the Human NF90 / ILF3 Sandwich ELISA Kit, supplemented with details on Storage, Product Description, Key Features, What's Included, and Applications:


Product Information: Human NF90 / ILF3 Sandwich ELISA Kit


Storage
- Temperature: Store the kit components at 2-8°C. Avoid freezing.
- Stability: The kit remains stable until the expiration date when stored and handled correctly.
- Specific Components: Some components may require different storage conditions (e.g., standard solutions), which will be specified in the kit instructions.


Product Description
- Overview: The Human NF90 / ILF3 Sandwich ELISA Kit is designed for the quantitative detection of NF90 / ILF3 protein levels in various biological samples, including serum, plasma, and cell culture supernatants.
- Principle: This ELISA is based on the sandwich principle, where the target protein is captured between two specific antibodies. The detection is facilitated via a colorimetric reaction measured at a specific wavelength.
- Sensitivity and Specificity: The kit offers high sensitivity and specificity due to the use of monoclonal antibodies.


Key Features
- High Sensitivity: Capable of detecting low concentrations of NF90 / ILF3.
- High Specificity: Minimizes cross-reactivity with other proteins.
- Quantitative Results: Provides accurate and reproducible measurements.
- User-Friendly Protocol: Designed for ease of use with step-by-step instructions.
- Fast Assay Time: Complete the assay in approximately 3-4 hours.


What's Included
- Microplate: Pre-coated with anti-NF90 / ILF3 capture antibody.
- Standards: Recombinant NF90 / ILF3 protein standards for generating a calibration curve.
- Detection Antibody: Biotinylated anti-NF90 / ILF3 antibody.
- Enzyme Conjugate: Streptavidin-HRP for signal development.
- Substrate Solution: TMB (Tetramethylbenzidine) solution for color development.
- Stop Solution: Typically an acidic solution to halt the enzyme reaction.
- Wash Buffer: Concentrated buffer for washing steps.
- Sample Diluent: Buffer for proper sample preparation.
- Instruction Manual: Detailed protocol and troubleshooting guide.


Applications
- Research: Ideal for studying NF90 / ILF3 in cellular processes, gene regulation, and immune responses.
- Disease Studies: Useful in investigating diseases where NF90 / ILF3 is implicated, such as cancer and autoimmune disorders.
- Biomarker Discovery: Facilitates the identification and validation of NF90 / ILF3 as a potential biomarker.
- Drug Development: Assists in evaluating the effects of therapeutic compounds on NF90 / ILF3 expression levels.

These elements provide a detailed understanding of the Human NF90 / ILF3 Sandwich ELISA Kit, ensuring researchers have the necessary information to effectively utilize the product in their studies.