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产品描述
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Unit Definitions
One unit of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutesat 37°C.
One unit of ScriptGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity ismeasured by the inhibition of hydrolysis of cyclic 2',3'-CMP by RNase A.
Functional Testing
The AmpliCap-Max T7 High Yield Message Maker Kit is functionally tested under standard reactionconditions using the T7 Control Template DNA. The kit must produce at least 40 µg of RNA from1 µg of the T7 Control Template DNA in 30 minutes at 37°C.
Contaminating Activity Assays
All components of the AmpliCap-Max T7 High Yield Message Maker Kit are free of detectable RNaseand DNase activity, except for the inherent activity of the RNase-Free DNase I component.
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