INCOGNITO™ T7 -RNA Transcription Kit,C-ICTY110510,CELLSCRIPT,安诺伦(北京)生物科技有限公司

Unit Definition
One unit of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutesat 37°C.
One unit of ScriptGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity ismeasured by the inhibition of hydrolysis of cyclic 2',3'-CMP by RNase A.
Functional Testing
The INCOGNITO T7 Ψ-RNA Transcription Kit is functionally tested under standard reactionconditions using the T7 Control Template DNA. The kit must produce at least 90 µg of RNA from1 µg of the T7 Control Template DNA in 2 hours at 37°C.
Contaminating Activity Assays
All components of the INCOGNITO T7 Ψ-RNA Transcription Kit are free of detectable RNase andDNase activity, except for the inherent activity of the RNase-Free DNase I component.