INCOGNITO™ T7 ARCA 5mC- & -RNA Transcription Kit,C-ICTAMY110510,CELLSCRIPT,安诺伦(北京)生物科技有限公司

Unit Definitions
One unit of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutesat 37°C.
One unit of ScriptGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity ismeasured by the inhibition of hydrolysis of cyclic 2',3'-CMP by RNase A.
Functional Testing
The INCOGNITO T7 ARCA 5mC- & Ψ-RNA Transcription Kit is functionally tested under standardreaction conditions using the T7 Control Template DNA. The kit must produce at least 35 µg of RNAfrom 1 µg of the T7 Control Template DNA in 3 hours at 37°C.
Contaminating Activity Assays
All components of the INCOGNITO T7 ARCA 5mC- & Ψ-RNA Transcription Kit are free of detectableRNase and DNase activity, except for the inherent activity of the RNase-Free DNase I component.