SP6-Scribe™ Standard RNA IVT Kit,C-AS3106,CELLSCRIPT,安诺伦(北京)生物科技有限公司

Unit Definition
One unit of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutesat 37°C.
One unit of ScriptGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity ismeasured by the inhibition of hydrolysis of cyclic 2',3'-CMP by RNase A.
Functional Testing
The SP6-Scribe Standard RNA IVT Kit is functionally tested under standard reaction conditions usingthe SP6 Control Template DNA. The kit must produce at least 80 µg of RNA from 1 µg of the SP6Control Template DNA in 2 hours at 37°C.
Contaminating Activity Assays
All components of the SP6-Scribe Standard RNA IVT Kit are free of detectable RNase and DNaseactivity, except for the inherent activity of the RNase-Free DNase I component.