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产品描述
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Certainly! Here's a detailed overview of the recombinant RFP (Red Fluorescent Protein) product information:
Storage
- Temperature: Store at -20°C or lower for long-term storage. For short-term use, it can be kept at 4°C.
- Conditions: Protect from light to prevent photobleaching. Ensure that the storage container is tightly sealed to avoid contamination and evaporation.
- Stability: When stored correctly, recombinant RFP remains stable for up to 12 months. Avoid repeated freeze-thaw cycles.
Product Description
- Origin: Recombinant RFP is derived from Discosoma species and optimized for enhanced fluorescence and stability.
- Molecular Weight: Approximately 27 kDa.
- Structure: Monomeric form, engineered for improved folding and brightness.
- Purity: ≥95% purity as determined by SDS-PAGE.
Key Features
- Brightness: Emits vibrant red fluorescence, ideal for imaging applications.
- Photostability: Enhanced resistance to photobleaching compared to traditional fluorescent proteins.
- pH Stability: Maintains fluorescence over a wide pH range, typically from pH 6 to pH 8.
- Versatility: Compatible with various biological assays, including live-cell imaging and flow cytometry.
What's Included
- Recombinant RFP: Supplied in a buffered solution to ensure stability.
- Documentation: Detailed user manual with protocols for application and troubleshooting.
- Certificate of Analysis: Verification of purity and activity.
Applications
- Fluorescence Microscopy: Ideal for studying cellular processes in real-time.
- Flow Cytometry: Suitable for single-cell analysis and sorting.
- Live-cell Imaging: Non-invasive tracking of gene expression and protein localization.
- FRET Studies: Can be paired with other fluorescent proteins for Förster Resonance Energy Transfer experiments.
- Reporter Assays: Useful in gene expression analysis, promoter activity studies, and other molecular biology applications.
By supplementing the product information with these details, users can better understand the storage needs, functionality, and applications of recombinant RFP, enhancing their experimental outcomes and ensuring optimal use of the product.
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