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产品描述
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Certainly! Here's a comprehensive overview of the pCMV-Ampho Envelope Vector, covering the suggested aspects:
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pCMV-Ampho Envelope Vector: Product Information
Storage
- Temperature: Store at -20°C for long-term stability.
- Handling: Avoid repeated freeze-thaw cycles to maintain product integrity.
- Packaging: Typically provided in a lyophilized form or as a plasmid DNA in suspension. Ensure the container is tightly closed and protected from light.
Product Description
- Overview: The pCMV-Ampho Envelope Vector is designed for use in retroviral packaging systems. It contains the amphotropic envelope gene under the control of the CMV promoter, enabling efficient pseudotyping of retroviral particles that can infect a wide range of mammalian cells.
- Vector Backbone: Incorporates high-efficiency elements for gene expression and packaging compatibility.
- Purpose: Ideal for creating stable producer cell lines for gene therapy or research applications involving retroviral vectors.
Key Features
- Broad Host Range: The amphotropic envelope allows for infection across diverse mammalian cell types.
- High Efficiency: Utilizes the CMV promoter for robust expression of the envelope protein.
- Versatility: Compatible with various retroviral systems, enhancing flexibility in experimental design.
- Safety: Designed for laboratory use with safety protocols in place to prevent unintended release or infection.
What's Included
- Vector Material: pCMV-Ampho Envelope plasmid DNA.
- Documentation: Detailed instruction manual including cloning and expression protocols.
- Support: Access to technical support for troubleshooting and optimization.
Applications
- Gene Therapy Research: Facilitates the delivery of therapeutic genes into target cells.
- Cell Line Development: Useful for generating stable cell lines that produce retroviral particles for further study.
- Basic Research: Investigate mechanisms of viral entry and infection in various cell types.
- Biotechnology: Enhance production of recombinant proteins or study gene expression in mammalian systems.
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This overview should serve as a useful guide for understanding, utilizing, and maximizing the benefits of the pCMV-Ampho Envelope Vector in various research and development applications.
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