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产品描述
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Certainly! Let's provide detailed information for the pCMV-Eco Envelope Vector, covering Storage, Product Description, Key Features, What's Included, and Applications.
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Storage
- Temperature: Store the pCMV-Eco Envelope Vector at -20°C for long-term stability.
- Handling: Avoid repeated freeze-thaw cycles to maintain vector integrity.
- Shelf Life: When stored correctly, the vector is stable for up to 12 months.
Product Description
The pCMV-Eco Envelope Vector is a plasmid designed for use in retroviral packaging systems, facilitating the production of high-titer viral particles. It encodes the ecotropic envelope protein, allowing for efficient transduction of mouse and other rodent cells. The CMV promoter ensures high-level expression of the envelope protein in mammalian cells.
Key Features
- High Efficiency: Optimized for robust production of ecotropic viral particles.
- CMV Promoter: Provides strong transcriptional activity in a wide range of mammalian cells.
- Ecotropic Specificity: Ideal for targeting rodent cell lines.
- Versatile Applications: Suitable for gene delivery, stable cell line creation, and functional studies.
What's Included
- Plasmid DNA: A vial containing a specified quantity of pCMV-Eco Envelope Vector.
- User Guide: Detailed instructions for use, including protocols for transfection and viral production.
- Certificate of Analysis: Provides data on the purity and concentration of the plasmid.
Applications
- Gene Delivery: Use in conjunction with retroviral systems to introduce genes into mouse or rat cells.
- Cell Line Development: Efficient generation of stable cell lines expressing the gene of interest.
- Research Studies: Ideal for investigating gene function, protein expression, and pathway analysis in rodent models.
- Viral Packaging: Essential component for producing ecotropic retroviral particles in packaging cell lines.
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These details should help in understanding the product and optimizing its use in laboratory settings. If you need further clarification on any aspect or have specific questions, feel free to ask!
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