RV-110,pCMV-VSV-G Retroviral Vector,Cell Biolabs

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Product Information: pCMV-VSV-G Retroviral Vector


Storage
- Temperature: Store at -20°C for long-term stability.
- Conditions: Keep the vector in a frost-free freezer to prevent degradation. Avoid repeated freeze-thaw cycles to maintain efficacy.
- Shelf Life: Typically stable for up to 12 months when stored properly. It's recommended to aliquot the vector upon first use to minimize freeze-thaw cycles.
- Handling: When thawing, maintain the vector on ice and use quickly to preserve activity.


Product Description
The pCMV-VSV-G Retroviral Vector is designed for efficient gene delivery and expression in a broad range of mammalian cells. Utilizing the vesicular stomatitis virus G (VSV-G) protein, this vector enhances tropism and improves transduction efficiency across various cell types, including both dividing and non-dividing cells. The CMV promoter ensures robust expression of the gene of interest.


Key Features
- Broad Tropism: VSV-G pseudotyping allows for transduction of a wide variety of mammalian cells.
- High Efficiency: Optimized vector design for maximum gene transfer efficiency.
- Versatile: Suitable for various applications including stable cell line creation and gene therapy research.
- Robust Expression: Features the CMV promoter for strong and consistent gene expression.
- Safety: Designed to minimize the risk of insertional mutagenesis.


What's Included
- pCMV-VSV-G Retroviral Vector: Supplied in a concentrated, ready-to-use format.
- User Guide: Comprehensive instructions for handling, transduction protocols, and troubleshooting.
- Technical Support: Access to expert support for optimizing experimental conditions and addressing queries.


Applications
- Gene Therapy Research: Ideal for exploring gene delivery mechanisms and therapeutic potential.
- Stable Cell Line Development: Efficiently generate stable cell lines expressing your gene of interest.
- Functional Genomics: Use in studies to understand gene function and regulation.
- Oncology Research: Investigate the effects of gene manipulation in cancer cell models.
- Neuroscience Studies: Explore gene function in neuronal cells due to the broad tropism of VSV-G.

By providing detailed information across these aspects, users can effectively utilize the pCMV-VSV-G Retroviral Vector for their experimental needs while ensuring proper handling and storage for optimal performance.