RV-100,293RTV Cell Line,Cell Biolabs

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Storage:
- Temperature: Store the 293RTV Cell Line in liquid nitrogen vapor to ensure viability and prevent degradation.
- Handling: Minimize freeze-thaw cycles, as repeated freezing and thawing can affect cell viability and function.
- Labeling: Clearly label storage containers with the cell line name, passage number, date of freezing, and any other relevant information for easy identification.

Product Description:
- The 293RTV Cell Line is derived from human embryonic kidney (HEK) cells and has been modified to express the simian virus 40 (SV40) large T antigen.
- This modification allows for efficient replication of plasmid vectors containing the SV40 origin of replication, making it ideal for high-yield protein production and viral packaging.
- The cells adhere easily to culture surfaces, growing in a monolayer with a doubling time of approximately 24 hours under optimal conditions.

Key Features:
- High Transfection Efficiency: The 293RTV Cell Line is highly amenable to transfection, facilitating the expression of recombinant proteins and viral vectors.
- Robust Growth: Exhibits rapid proliferation and stable growth characteristics, ideal for laboratory scale-up.
- Versatility: Suitable for a wide range of applications, including gene expression studies, viral production, and protein purification.
- Stable Expression: Supports stable and transient expression systems, providing flexibility for various research needs.

What's Included:
- Frozen Vial: A cryovial containing approximately 1 million 293RTV cells, shipped on dry ice.
- Certificate of Analysis: Documentation verifying the cell line's identity, purity, and performance characteristics.
- User Manual: Detailed instructions for thawing, culturing, and maintaining the cell line for optimal results.
- Technical Support: Access to expert advice and troubleshooting assistance from the supplier.

Applications:
- Protein Production: Ideal for the production of recombinant proteins, including antibodies and therapeutic enzymes.
- Viral Vector Packaging: Commonly used in the production of adenoviral and lentiviral vectors for gene therapy research.
- Biomedical Research: Supports studies in gene regulation, signal transduction, and cancer research.
- Vaccine Development: Utilized in the creation and testing of viral vaccines, especially for adenovirus-based vectors.
- Drug Discovery: Provides a robust platform for high-throughput screening and drug development assays.

By thoroughly detailing these aspects, you can ensure that potential users have a clear understanding of the cell line's capabilities and uses, ultimately enhancing the appeal and utility of the 293RTV Cell Line for research purposes.