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产品描述
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Certainly! Here's a structured and detailed supplement to the product information for the pMXs-EF1-Bsd Retroviral Vector:
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Product Information for pMXs-EF1-Bsd Retroviral Vector
Storage
- Temperature: Store the vector at -20°C for long-term storage.
- Avoid Freeze/Thaw Cycles: Minimize repeated freeze-thaw cycles to maintain vector integrity.
- Stability: When stored properly, the vector remains stable for at least one year.
Product Description
The pMXs-EF1-Bsd Retroviral Vector is a highly efficient tool designed for gene delivery and stable integration into host cell genomes. This retroviral vector uses the EF1 promoter, known for its robust and ubiquitous expression, to drive the expression of the gene of interest. The vector also includes a Blasticidin resistance gene for selection purposes. It is suitable for a variety of applications including gene function studies, stable cell line generation, and therapeutic research.
Key Features
- Efficient Transduction: Achieves high transduction efficiency in dividing cells.
- Stable Integration: Facilitates stable gene expression due to integration into host genome.
- Selectable Marker: Contains a Blasticidin resistance gene for easy selection of successfully transduced cells.
- Strong Promoter: EF1 promoter ensures consistent and strong expression across different cell types.
- Versatile: Compatible with a wide range of mammalian cells.
What's Included
- Vector Plasmid: pMXs-EF1-Bsd plasmid DNA, typically provided in a concentration suitable for transfection.
- Detailed Protocol: Instructions for vector use, including transfection and selection procedures.
- Safety Data Sheet (SDS): Information on handling and potential hazards.
- Technical Support: Access to support for troubleshooting and optimization.
Applications
- Gene Function Studies: Analyze gene function through stable expression in target cells.
- Stable Cell Line Generation: Create stable cell lines for long-term experiments.
- Therapeutic Research: Explore gene therapy applications and develop potential treatments.
- Cancer Research: Investigate oncogenes or tumor suppressor genes by stable expression in cancer cell models.
- Biomarker Discovery: Use for the identification and validation of biomarkers in various cell types.
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This detailed product information aims to provide comprehensive guidance for researchers utilizing the pMXs-EF1-Bsd Retroviral Vector in their studies.
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