RTV-060,pMXs-EF1-Puro Retroviral Vector,Cell Biolabs

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Product Name: pMXs-EF1-Puro Retroviral Vector

Storage:
- Temperature: The vector should be stored at -20°C to maintain stability over time. For long-term storage, -80°C is recommended.
- Conditions: Avoid repeated freeze-thaw cycles to preserve the integrity of the plasmid DNA.
- Packaging: Ensure the vector is stored in a tightly sealed container or vial to prevent contamination and moisture ingress.

Product Description:
- The pMXs-EF1-Puro Retroviral Vector is a widely used tool for gene delivery in mammalian cells. This vector is based on the Moloney Murine Leukemia Virus (MMLV) and is designed for high-efficiency transduction.
- It contains the EF1α promoter, which allows robust and stable expression of the inserted gene across various cell types.
- The vector includes a puromycin resistance gene, enabling easy selection of successfully transduced cells with puromycin.

Key Features:
- High Efficiency: Capable of efficient transduction in a broad range of mammalian cell lines.
- Stable Expression: Utilizes the EF1α promoter for consistent expression levels.
- Selectable Marker: Incorporates a puromycin resistance gene for straightforward selection of transduced cells.
- Versatile Applications: Suitable for both transient and stable gene expression studies.

What's Included:
- Plasmid DNA: The vector is supplied in a sufficient quantity for multiple transduction experiments.
- User Manual: Detailed instructions for vector use, including protocols for transfection and puromycin selection.
- Technical Support: Access to expert technical support for troubleshooting and experimental design assistance.

Applications:
- Gene Overexpression: Ideal for overexpressing genes of interest to study their function in mammalian cells.
- Stable Cell Line Generation: Facilitates the creation of stable cell lines through puromycin selection.
- Functional Genomics: Useful for functional genomics studies, including gene knockdown, knockout, and rescue experiments.
- Cancer Research: Enables the study of oncogenes and tumor suppressor genes in cancer cell models.
- Cellular Reprogramming: Can be used in protocols for cellular reprogramming and stem cell research.

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