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产品描述
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Certainly! Below is a detailed product information guide for the pMX-GFP Retroviral Vector, covering the sections you mentioned:
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Product Name: pMX-GFP Retroviral Vector
Storage:
- Temperature: Store at -20°C for long-term storage.
- Conditions: Avoid repeated freeze-thaw cycles to maintain vector integrity.
- Shelf Life: Typically stable for 6-12 months when stored correctly.
Product Description:
The pMX-GFP Retroviral Vector is a high-efficiency tool designed for the stable integration and expression of the GFP gene in various mammalian cells. It utilizes the Moloney Murine Leukemia Virus (MMLV) backbone, optimized for effective transduction and robust gene expression. The vector is engineered to allow for easy tracking of transduced cells via GFP fluorescence.
Key Features:
- High Transduction Efficiency: Ensures reliable gene delivery into target cells.
- GFP Reporter: Facilitates easy visualization and tracking of transduced cells using fluorescence microscopy.
- Broad Host Range: Suitable for a wide variety of mammalian cell types.
- Stability: Provides stable gene expression through integration.
- Safety: Contains enhanced safety features, such as self-inactivating (SIN) sequences, to minimize undesired replication.
What's Included:
- pMX-GFP Plasmid DNA: Supplied in a lyophilized or liquid format at a specified concentration.
- Detailed Protocol: Instructions for vector production, transduction, and expression analysis.
- Safety Data Sheet (SDS): Provides guidelines for safe handling and use.
- Technical Support: Access to expert advice for troubleshooting and optimization.
Applications:
- Cell Labeling: Use GFP fluorescence for visualizing and tracking cell populations in real-time.
- Gene Function Studies: Analyze the effects of gene expression in various cellular contexts.
- Developmental Biology: Study cellular differentiation and lineage tracing.
- Cancer Research: Investigate the role of specific genes in oncogenesis and tumor progression.
- Gene Therapy Research: Explore potential therapeutic applications and gene delivery strategies.
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For optimal use of the pMX-GFP Retroviral Vector, it is recommended that researchers follow the provided protocol and guidelines carefully, ensuring the maintenance of sterile conditions to prevent contamination. Additionally, users should take appropriate safety precautions when handling retroviral vectors to ensure safe and effective experimentation.
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