RTV-021,pMYs-IRES-GFP Retroviral Vector,Cell Biolabs

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Product Name:
pMYs-IRES-GFP Retroviral Vector

Storage:
- Store at -80°C for long-term stability.
- Avoid repeated freeze-thaw cycles to maintain vector integrity.
- For short-term use, vectors can be stored at 4°C for up to one week.
- Ensure the storage area is free from contaminants and maintain a stable temperature.

Product Description:
The pMYs-IRES-GFP Retroviral Vector is a versatile tool designed for gene delivery and expression in mammalian cells. This vector facilitates the insertion of a gene of interest alongside the GFP reporter, enabling easy visualization and tracking of transduction efficiency. The Internal Ribosome Entry Site (IRES) allows for bicistronic expression, ensuring simultaneous translation of the gene of interest and GFP within the same cellular context.

Key Features:
- Bicistronic Expression: Incorporates IRES for dual expression of the target gene and GFP, allowing for efficient monitoring.
- High Efficiency: Optimized for transduction in a variety of mammalian cell lines.
- GFP Reporter: Enables direct visualization and quantification of transduction efficiency via fluorescence microscopy.
- Safety Features: Contains safety modifications to reduce the risk of replication-competent retrovirus formation.

What's Included:
- pMYs-IRES-GFP Retroviral Vector Plasmid: A vial containing a specified concentration and volume of the vector.
- Detailed Protocol: Step-by-step instructions for transfection and transduction procedures.
- Quality Control Certificate: Documentation ensuring vector integrity and performance.
- Technical Support: Access to expert guidance for troubleshooting and optimization.

Applications:
- Gene Therapy Research: Ideal for delivering therapeutic genes in preclinical studies.
- Cell Line Engineering: Useful in the development of stable cell lines expressing recombinant proteins.
- Functional Genomics Studies: Facilitates the study of gene function and regulation in a controlled environment.
- Fluorescence-Based Assays: Suitable for applications requiring GFP-based visualization, such as live-cell imaging.

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