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产品描述
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Certainly! Here is a structured supplement and improvement for the product information regarding the pMYs-IRES-Puro Retroviral Vector:
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Product Name: pMYs-IRES-Puro Retroviral Vector
Storage:
- Store at -20°C for long-term preservation.
- Avoid repeated freeze-thaw cycles to maintain vector integrity.
- If thawed, keep on ice and use promptly.
Product Description:
The pMYs-IRES-Puro Retroviral Vector is a versatile genetic tool designed for efficient gene delivery and stable expression in mammalian cells. This retroviral vector facilitates the integration of the gene of interest into the host genome, ensuring stable and long-term expression. The IRES (Internal Ribosome Entry Site) allows for bicistronic expression, enabling simultaneous translation of two genes from a single mRNA transcript. The puromycin resistance gene provides a selection marker for successful transduction events.
Key Features:
- Bicistronic Expression: Enables simultaneous expression of your gene of interest and a selectable marker.
- Puromycin Resistance: Facilitates easy selection of transduced cells.
- Stable Integration: Retroviral integration ensures long-term gene expression.
- High Efficiency: Designed for efficient transduction across a wide range of cell types.
What's Included:
- pMYs-IRES-Puro Retroviral Vector plasmid DNA (sufficient quantity for multiple transductions).
- Detailed protocol for transfection and selection.
- Technical support for troubleshooting and optimization.
Applications:
- Gene Function Studies: Ideal for examining gene function and regulation in various mammalian cell models.
- Drug Screening: Utilize puromycin selection to create stable cell lines for high-throughput drug screening.
- Cancer Research: Study oncogenes and tumor suppressor genes in cancer cell lines.
- Cell Line Engineering: Create custom cell lines for research and biotechnological applications.
- Protein Expression: Efficient expression of recombinant proteins for biochemical and structural studies.
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Feel free to reach out if you need further customization or additional details on any specific aspect!
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